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Human XPO1 (Exportin 1) ELISA Kit

Human XPO1 (Exportin 1) ELISA Kit

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Catalogue Number: ELK4663

Alternative Names: Exp1; CRM1 Homolog,Yeast; Chromosome region maintenance 1 protein homolog

Species: Human

Assay Type: Sandwich

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research: Signal transduction;Infection immunity;Immunodeficiency;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Exportin 1(XPO1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Exportin 1(XPO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Exportin 1(XPO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Exportin 1(XPO1) in the samples is then determined by comparing the OD of the samples to the standard curve.