Catalogue Number: ELK4469
Alternative Names: KARP1; KU80; KUB2; Ku86; NFIV; TLAA; Lupus Ku Autoantigen Protein P80; Nuclear factor IV; 86 kDa of Ku antigen; CTC box-binding factor 85 kDa; Thyroid-lupus autoantigen
Species: Human
Assay Type: Sandwich
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research: Tumor immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to X-Ray Repair Cross Complementing 5(XRCC5). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to X-Ray Repair Cross Complementing 5(XRCC5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain X-Ray Repair Cross Complementing 5(XRCC5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of X-Ray Repair Cross Complementing 5(XRCC5) in the samples is then determined by comparing the OD of the samples to the standard curve.