Catalogue Number: ELK4710
Alternative Names: SLC7A7; LPI; Y+LAT1; MOP-2; Monocyte amino acid permease 2; Y+L Amino Acid Transporter 1; Solute Carrier Family 7 Member 7,Cationic Amino Acid Transporter,y+ System
Species: Human
Assay Type: Sandwich
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research: Signal transduction;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to L-Type Amino Acid Transporter 1(y+LAT1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to L-Type Amino Acid Transporter 1(y+LAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain L-Type Amino Acid Transporter 1(y+LAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of L-Type Amino Acid Transporter 1(y+LAT1) in the samples is then determined by comparing the OD of the samples to the standard curve.