{"product_id":"hvd3-25-hydroxyvitamin-d3-elisa-kit-elk7890","title":"HVD3 (25-Hydroxyvitamin D3) ELISA Kit - ELK7890","description":"\u003cp\u003eHVD3 (25-Hydroxyvitamin D3) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK7890-48, ELK7890-96, ELK7890-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: 25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25 (OH)D\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.91 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 200 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 3.13-200 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Reproductive science; Genetic science; Nutrition metabolism; Bone metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HVD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43212239995059,"sku":"ELK7890-48","price":371.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43212240027827,"sku":"ELK7890-96","price":526.4,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200786096307,"sku":"ELK7890-5x96","price":2237.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_a901bf91-11b8-4a4c-978f-af6a29b1a9ff.png?v=1767063744","url":"https:\/\/afsbio.com\/products\/hvd3-25-hydroxyvitamin-d3-elisa-kit-elk7890","provider":"AFSBio Inc.","version":"1.0","type":"link"}