{"product_id":"hyp-hydroxyproline-elisa-kit-elk7835","title":"Hyp (Hydroxyproline) ELISA Kit - ELK7835","description":"\u003cp\u003eHyp (Hydroxyproline) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK7835-48, ELK7835-96, ELK7835-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names:  (2S,4R)-4-Hydroxyproline; L-Hydroxyproline (C5H9O3N)\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 25.4 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 5000 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 78.13-5000 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Metabolic pathway; Nutrition metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hyp. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hyp. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hyp in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43212152996019,"sku":"ELK7835-48","price":371.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43212153028787,"sku":"ELK7835-96","price":526.4,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200764272819,"sku":"ELK7835-5x96","price":2237.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_0e006022-d8fe-45c7-8d16-5cb2e4bbd536.png?v=1767063676","url":"https:\/\/afsbio.com\/products\/hyp-hydroxyproline-elisa-kit-elk7835","provider":"AFSBio Inc.","version":"1.0","type":"link"}