{"product_id":"il-2rb-cd122-recombinant-protein-ncp0240","title":"IL-2RB\/CD122 Recombinant Protein - NCP0240","description":"\u003cp\u003eIL-2RB\/CD122 Recombinant Protein\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: NCP0240-500, NCP0240-1000\u003c\/p\u003e\n\n\u003cp\u003eSizes: 500ug, 1mg\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P14784\u003c\/p\u003e\n\n\u003cp\u003eHost: E.coli\u003c\/p\u003e\n\n\u003cp\u003eTag: His-tag\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: GCAGTGAATGGCACCAGTCAGTTCACCTGCTTCTATAATAGTCGTGCCAATATTAGTTGTGTGTGGAGTCAGGATGGCGCACTGCAGGATACCAGTTGCCAGGTTCATGCCTGGCCGGATCGCCGTCGTTGGAATCAGACCTGTGAACTGCTGCCGGTGAGTCAGGCAAGTTGGGCATGTAATCTGATTCTGGGCGCCCCGGATAGTCAGAAACTGACCACCGTTGATATTGTTACCCTGCGCGTTCTGTGCCGCGAAGGCGTTCGTTGGCGCGTGATGGCCATTCAGGACTTCAAACCGTTCGAAAATCTGCGTCTGATGGCACCGATTAGTCTGCAGGTGGTGCATGTGGAAACACATCGTTGTAATATTAGTTGGGAAATTAGTCAGGCCAGCCATTACTTCGAACGCCATCTGGAATTCGAAGCCCGCACCCTGAGCCCGGGCCATACCTGGGAAGAAGCACCGCTGCTGACCCTGAAACAGAAACAGGAATGGATCTGTCTGGAAACCTTAACCCCGGATACCCAGTATGAATTCCAGGTGCGCGTGAAACCGCTGCAGGGCGAATTCACCACCTGGAGCCCGTGGAGTCAGCCGCTGGCATTCCGCACCAAACCGGCAGCACTGGGTAAAGATACC\u003c\/p\u003e\n\n\u003cp\u003eRestriction Sites: NdeI-XhoI\u003c\/p\u003e\n\n\u003cp\u003eBackground: Interleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation. IL-2 also promotes peripheral development of regulatory T cells (Tregs) . Conversely, IL-2 is involved in the activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells. The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ, and the common gamma chain, γc. IL-2Rα binds exclusively to IL-2 with low affinity and increases the binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-15 also binds to IL-2Rβ. γc is used by other cytokines including IL-4, IL-7, IL-9, IL-15, and IL-21. Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K\/Akt pathways. IL-2Rβ, also known as CD122, is a type I transmembrane protein that is expressed by natural killer cells and certain subsets of T cells in the periphery. IL-2Rβ surface expression is upregulated following antigen-induced activation. A heteromeric complex composed of IL-2Rβ and γc forms the intermediate affinity IL-2 receptor whereas a complex comprised of all three chains forms the high affinity receptor. Signaling through IL-2Rβ is mediated by Jak1, which phosphorylates numerous tyrosine residues in the cytoplasmic tail to promote the recruitment of the adaptor Shc to IL-2Rβ and enhance IL-2 receptor signaling.\u003c\/p\u003e\n\n\u003cp\u003eSoluble: PBS, 4M Urea, PH7.4\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is \u0026gt; 85% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eExpression Vector: pet-22b(+)\u003c\/p\u003e\n\n\u003cp\u003eBioWorld Molecular Weight: ~26kDa\u003c\/p\u003e\n\n\u003cp\u003eNotes: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"500ug","offer_id":42887062094003,"sku":"NCP0240-500","price":1050.83,"currency_code":"CAD","in_stock":true},{"title":"1mg","offer_id":42887062126771,"sku":"NCP0240-1000","price":1857.89,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_42e9bbd8-ee66-4f0d-a3a0-8874c2bcc548.jpg?v=1702317301","url":"https:\/\/afsbio.com\/products\/il-2rb-cd122-recombinant-protein-ncp0240","provider":"AFSBio Inc.","version":"1.0","type":"link"}