{"product_id":"il-4rα-phospho-tyr497-rabbit-polyclonal-antibody-es5879","title":"IL-4Rα (phospho Tyr497) Rabbit Polyclonal Antibody - ES5879","description":"\u003cp\u003eIL-4Rα (phospho Tyr497) Rabbit Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES5879-50, ES5879-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Polymorphisms in this gene are also associated with resistance to human immunodeficiency virus type-1 infection. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012],\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF, ELISA\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: IL4R, IL4RA, 582J2.1, Interleukin-4 receptor subunit alpha, IL-4 receptor subunit alpha, IL-4R subunit alpha, IL-4R-alpha, IL-4RA, CD antigen CD124\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human IL-4R\/CD124 around the phosphorylation site of Tyr497. AA range:463-512\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eTarget: IL-4R\/CD124\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Phospho-IL-4Rα (Y497) Polyclonal Antibody detects endogenous levels of IL-4Rα protein only when phosphorylated at Y497.\u003c\/p\u003e\n\n\u003cp\u003eDilution: Western Blot: 1\/500 - 1\/2000. Immunohistochemistry: 1\/100 - 1\/300. Immunofluorescence: 1\/200 - 1\/1000. ELISA: 1\/5000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml,\u003c\/p\u003e\n\n\u003cp\u003eFormlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eExpression: Isoform 1 and isoform 2 are highly expressed in activated T-cells,\u003c\/p\u003e\n\n\u003cp\u003eObserved Band (KD): 90kD\u003c\/p\u003e\n\n\u003cp\u003eFields: Cytokine-cytokine receptor interaction, PI3K-Akt signaling pathway, JAK-STAT signaling pathway, Hematopoietic cell lineage, Th1 and Th2 cell differentiation, Th17 cell differentiation, Pathways in cancer, Inflammatory bowel disease\u003c\/p\u003e\n\n\u003cp\u003eFunction: Domain: Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases\u003cbr\u003e\nDomain: The box 1 motif is required for JAK interaction and\/or activation\u003cbr\u003e\nDomain: The extracellular domain represents the IL4 binding protein (IL4BP)\u003cbr\u003e\nDomain: The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding\u003cbr\u003e\nFunction: Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1\/2\/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4\/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammmation. In certain cel\u003c\/p\u003e\n\n\u003cp\u003eSubcellular Location: Cell membrane, Single-pass type I membrane protein, [Isoform 2]: Secreted\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eGene Name: IL4R\u003c\/p\u003e\n\n\u003cp\u003eProtein Name: Interleukin-4 receptor subunit alpha\u003c\/p\u003e\n\n\u003cp\u003eMouse Gene ID: 16190\u003c\/p\u003e\n\n\u003cp\u003eMouse SwissProt Number: P16382\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":46313572434099,"sku":"ES5879-50","price":186.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":46313572466867,"sku":"ES5879-100","price":372.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/elk_antibodies_d75295e2-ffc1-4e0f-a3e7-87715c8983a9.jpg?v=1793190507","url":"https:\/\/afsbio.com\/products\/il-4r%ce%b1-phospho-tyr497-rabbit-polyclonal-antibody-es5879","provider":"AFSBio Inc.","version":"1.0","type":"link"}