{"product_id":"il6-monoclonal-antibody-mb9296","title":"IL6 Monoclonal Antibody-MB9296","description":"\u003cp\u003eIL6 Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB9296-50, MB9296-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Mouse IgG2b, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P05231(Human) P08505(Mouse) P20607(Rat)\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500-1:2,000\u003cbr\u003e\nICC:1:50-1:200\u003cbr\u003e\nIHC:1:50-1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Interleukin-6, or IL-6, is a multifunctional protein, 212 amino acids in length, that plays critical roles in host defense, immune response and hematopoiesis. IL-6 is constitutively expressed by epidermal Langerhans cells and its expression is induced in stimulated keratinocytes. IL-6, IL-1β and TNFα act as endogenous pyrogens, regulating the fever response to bacterial invasion. The IL-6 receptor is a trimeric complex composed of an IL-6-specific α chain and a homodimer of the gp130 glycoprotein common to the IL-6, IL-11, CNTF, OSM and LIF receptors. Stimulation with IL-6 leads to gp130 homodimerization and the activation of associated kinases JAK1 and JAK2. Once activated, JAK1 and JAK2 phosphorylate Stat3, causing its nuclear translocation and transcription of Stat3-responsive genes. IL-6 has also been shown to activate the Ras\/MAP kinase pathway, which regulates NFIL6 transcription.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProG affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: IL6 monoclonal antibody detects endogenous levels of IL6 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 24 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of IL6 on recombinant protein lysate using anti-IL6 antibody at 1\/1,000 dilution., ICC staining IL6 (green) in PC-3M cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: IL-6, BSF-2, CDF, IFN-beta-2, IL6, IFNB2, Interleukin BSF 2, B cell differentiation factor, B cell stimulatory factor 2, B-cell stimulatory factor 2, BSF 2, BSF-2, BSF2, CDF, CTL differentiation factor, Hepatocyte stimulatory factor, HGF, HSF, Hybridoma growth factor, Hybridoma growth factor Interferon beta-2, IFN-beta-2, IFNB2, IL 6, IL-6, IL6, IL6, Interferon beta 2, Interferon beta-2, Interleukin 6, Interleukin 6 (interferon beta 2), Interleukin BSF 2, Interleukin-6,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: Recombinant protein\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42987075600563,"sku":"MB9296-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42987075633331,"sku":"MB9296-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_3aee5cc4-8371-4142-8033-e82f05bb6706.jpg?v=1706008615","url":"https:\/\/afsbio.com\/products\/il6-monoclonal-antibody-mb9296","provider":"AFSBio Inc.","version":"1.0","type":"link"}