{"product_id":"il6-polyclonal-antibody-bs62100","title":"IL6 Polyclonal Antibody-BS62100","description":"\u003cp\u003eIL6 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS62100-50, BS62100-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P05231\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Rat, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Acute phase response is induced by interleukin-6 (IL-6) produced by T cells, macrophages, fibroblasts, endothelial and other cells . IL-6 induces proliferation or differentiation in many cell types including B cells, thymocytes and T cells. IL-6, in concert with TGF-β, is important for developing Th17 responses. IL-6 binds to IL-6Rα and through this association induces gp130 homodimerization . gp130 homodimerization triggers the Jak\/Stat cascade and the SHP-2\/Erk MAP kinase cascade . IL-6 also forms a complex with an IL-6Rα splice variant that is nonmembrane-associated . The IL-6\/soluble IL-6Rα complex can then activate the gp130 signaling pathway in cells that express gp130 but not IL-6Rα . Research studies have shown that IL-6, through increasing expression of proangiogenic VEGF, may also contribute to metastatic breast cancer.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 96% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: IL6 polyclonal antibody detects endogenous levels of IL6 protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of IL6 polyclonal antibody at 1:500 dilution\u003cbr\u003e\nLane1:Hela whole cell lysate(40ug)\u003cbr\u003e\nLane2:U-87MG whole cell lysate(40ug)\u003cbr\u003e\nLane3:HuT78 whole cell lysate(40ug)\u003cbr\u003e\nLane4:A549 whole cell lysate(40ug)\u003cbr\u003e\nLane5:MCF-7 whole cell lysate(40ug)\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 28 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Interleukin-6; IL-6; B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2; IL6; IFNB2\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to Human IL6.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42910467326131,"sku":"BS62100-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42910467358899,"sku":"BS62100-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_e5f0a582-1e07-4bf5-b687-5a6d95d13835.jpg?v=1703092965","url":"https:\/\/afsbio.com\/products\/il6-polyclonal-antibody-bs62100","provider":"AFSBio Inc.","version":"1.0","type":"link"}