{"product_id":"il7r-cd127-polyclonal-antibody-ncp0242p","title":"IL7R\/CD127 Polyclonal Antibody-NCP0242P","description":"\u003cp\u003eIL7R\/CD127 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: NCP0242P-50, NCP0242P-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P16871\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:5000~1:10000\u003c\/p\u003e\n\n\u003cp\u003eBackground: The IL-7 receptor (IL-7R) is comprised of two protein subunits, CD127\/IL-7Rα (IL-7Ralpha) and the common gamma chain (CD132), which is the major signaling component for several cytokines including IL-2, IL-4, IL-9, IL-15, and IL-21. CD127\/IL-7Rα is a transmembrane protein belonging to the cytokine receptor homology class 1 (CRH1) and is expressed by a wide variety of cells including immature B cells, thymic natural killer cells, bone marrow stromal cells, and T cells. On its own, CD127\/IL-7Rα functions as a receptor for two cytokine receptor complex signaling cascades: IL-7 and thymic stromal lymphopoietin (TSLP). IL-7 signaling contributes to T cell development and homeostasis whereas TSLP receptor signaling contributes to dendritic cell activation and B cell development.  IL-7 signaling is an essential component in regulating the homeostasis of naive and memory T cells as differential expression of CD127\/IL-7Rα is observed on naive and activated T cells, which occurs following TCR activation. Specifically, CD127\/IL-7Rα expression is downregulated on activated T cells and the subsequent re-expression of CD127\/IL-7Rα on these cells is indicative of cells that will differentiate into memory T cells. The A7R34 antibody is widely used to identify CD127\/IL-7Rα expression on both B and T cells.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: IL7R\/CD127 polyclonal antibody detects endogenous levels of IL7R\/CD127 protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of IL7R\/CD127 polyclonal antibody at 1:5000 dilution\u003cbr\u003e\nLane1:BV2 whole cell lysate(30ug)\u003cbr\u003e\nLane2:Jurkat whole cell lysate(30ug)\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 65 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; CD127; Il7r\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant protein of human IL7R\/CD127.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42908701229235,"sku":"NCP0242P-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42908701262003,"sku":"NCP0242P-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_1e44e6d6-b9b1-4873-9b60-1b691c68566d.jpg?v=1703005991","url":"https:\/\/afsbio.com\/products\/il7r-cd127-polyclonal-antibody-ncp0242p","provider":"AFSBio Inc.","version":"1.0","type":"link"}