{"product_id":"importin-9-rabbit-monoclonal-antibody-mb66257","title":"Importin 9 Rabbit Monoclonal Antibody-MB66257","description":"\u003cp\u003eImportin 9 Rabbit Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB66257-50, MB66257-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q96P70\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB (1\/500 - 1\/1000), IF\/ICC (1\/50 - 1\/100), IP (1\/10 - 1\/50)\u003c\/p\u003e\n\n\u003cp\u003eBackground: Nuclear transport receptor that mediates nuclear import of proteins, such as histones, proteasome and actin.\u003c\/p\u003e\n\n\u003cp\u003eServes as receptor for nuclear localization signals (NLS) in cargo substrates.\u003c\/p\u003e\n\n\u003cp\u003eIs thought to mediate docking of the importin\/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism.\u003c\/p\u003e\n\n\u003cp\u003eAt the nucleoplasmic side of the NPC, Ran binds to the importin, the importin\/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran.\u003c\/p\u003e\n\n\u003cp\u003eThe directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.\u003c\/p\u003e\n\n\u003cp\u003eMediates the import of pre-assembled proteasomes into the nucleus; AKIRIN2 acts as a molecular bridge between IPO9 and the proteasome complex.\u003c\/p\u003e\n\n\u003cp\u003eMediates the nuclear import of histones H2A, H2B, H4 and H4.\u003c\/p\u003e\n\n\u003cp\u003eIn addition to nuclear import, also acts as a chaperone for histones by preventing inappropriate non-nucleosomal interactions.\u003c\/p\u003e\n\n\u003cp\u003eMediates the nuclear import of actin.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was purified by immunogen affinity chromatography.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of Importin 9 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 120 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of Importin 9 expression in Jurkat (A), Hela (B) whole cell lysates., Immunofluorescent analysis of Importin 9 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: IMP9; KIAA1192; RANBP9; Importin-9; Imp9; Ran-binding protein 9; RanBP9\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic peptide of human Importin 9\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42987095130291,"sku":"MB66257-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42987095163059,"sku":"MB66257-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_741e69fe-7632-4357-a7a0-9c786a34f416.jpg?v=1706009088","url":"https:\/\/afsbio.com\/products\/importin-9-rabbit-monoclonal-antibody-mb66257","provider":"AFSBio Inc.","version":"1.0","type":"link"}