{"product_id":"instant-elisa-kit-for-follicle-stimulating-hormone-fsh-iea830hu","title":"Instant ELISA Kit for Follicle Stimulating Hormone (FSH) - IEA830Hu","description":"\u003cp\u003eInstant ELISA Kit for Follicle Stimulating Hormone (FSH)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 24T, 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: IEA830Hu-24, IEA830Hu-48, IEA830Hu-96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eFeatured Series: Instant kit\u003c\/p\u003e\n\n\u003cp\u003eTarget: Follicle Stimulating Hormone\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eUniprot: P01215\u003c\/p\u003e\n\n\u003cp\u003eGene ID: 1081\u003c\/p\u003e\n\n\u003cp\u003eFeatured Series Function: Detects protein (quick procedure in 1 hrs)\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Reactive with Human FSH \/ Follicle Stimulating Hormone\u003c\/p\u003e\n\n\u003cp\u003eMethod: Colormetric\u003c\/p\u003e\n\n\u003cp\u003eDetection principle: Double-antibody Sandwich\u003c\/p\u003e\n\n\u003cp\u003eDetection\u003cbr\u003e\nrange: 0.78-50mIU\/mL\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.31mIU\/mL\u003c\/p\u003e\n\n\u003cp\u003eAssay Time: 1h, 10min\u003c\/p\u003e\n\n\u003cp\u003eSample Size: 100uL\u003c\/p\u003e\n\n\u003cp\u003eRecommended\/Predicted\u003cbr\u003e\nSample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Precision: Intra-Assay: CV\u0026lt;10%, Inter-Assay: CV\u0026lt;12%\u003c\/p\u003e\n\n\u003cp\u003eStorage: 4°C for 1 month\/ -20°C for long-term(One year within shelf life)\u003c\/p\u003e\n\n\u003cp\u003eShelf-life: 12 months\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This assay has high sensitivity and excellent specificity for detection of Instant Follicle Stimulating Hormone (FSH).\u003cbr\u003e\nNo significant cross-reactivity or interference between Instant Follicle Stimulating Hormone (FSH) and analogues was observed.\u003c\/p\u003e\n\n\u003cp\u003ePrecision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Follicle Stimulating Hormone (FSH) were tested 20 times on one plate, respectively.\u003cbr\u003e\nInter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Follicle Stimulating Hormone (FSH) were tested on 3 different plates, 8 replicates in each plate.\u003cbr\u003e\nCV(%) = SD\/meanX100\u003c\/p\u003e\n\n\u003cp\u003eStability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.\u003cbr\u003e\nTo minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\u003c\/p\u003e\n\n\u003cp\u003eTarget: 1. Prepare all reagents, samples and standards;\u003cbr\u003e\n2. Add 50μL standard or sample and 50μL Detection Reagent A to each well. Incubate 1 hours at 37°C;\u003cbr\u003e\n3. Aspirate and wash 3 times;\u003cbr\u003e\n4. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37°C;\u003cbr\u003e\n5. Add 50μL Stop Solution. Read at 450nm immediately.\u003c\/p\u003e\n\n\u003cp\u003eTest principle: The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Follicle Stimulating Hormone (FSH). Standards or samples and HRP-labeled detection antibody specific to Instant Follicle Stimulating Hormone (FSH) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant Follicle Stimulating Hormone (FSH), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant Follicle Stimulating Hormone (FSH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Endocrinology; Reproductive science; Genetic science; Hormone metabolism;\u003c\/p\u003e \n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Cloud-Clone","offers":[{"title":"24T","offer_id":45379795189939,"sku":"IEA830Hu-24","price":336.0,"currency_code":"CAD","in_stock":true},{"title":"48T","offer_id":45379795222707,"sku":"IEA830Hu-48","price":588.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45379795255475,"sku":"IEA830Hu-96","price":840.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Cloud-Clone.jpg?v=1774117629","url":"https:\/\/afsbio.com\/products\/instant-elisa-kit-for-follicle-stimulating-hormone-fsh-iea830hu","provider":"AFSBio Inc.","version":"1.0","type":"link"}