{"product_id":"instant-elisa-kit-for-procollagen-i-n-terminal-propeptide-pinp-iea957ra","title":"Instant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP) - IEA957Ra","description":"\u003cp\u003eInstant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 24T, 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: IEA957Ra-24, IEA957Ra-48, IEA957Ra-96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eFeatured Series: Instant kit\u003c\/p\u003e\n\n\u003cp\u003eTarget: Procollagen I N-Terminal Propeptide\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Rat\u003c\/p\u003e\n\n\u003cp\u003eUniprot: P02454\u003c\/p\u003e\n\n\u003cp\u003eGene ID: 29393\u003c\/p\u003e\n\n\u003cp\u003eFeatured Series Function: Detects protein (quick procedure in 1 hrs)\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Reactive with Rat PINP \/ Procollagen I N-Terminal Propeptide\u003c\/p\u003e\n\n\u003cp\u003eMethod: Colormetric\u003c\/p\u003e\n\n\u003cp\u003eDetection principle: Double-antibody Sandwich\u003c\/p\u003e\n\n\u003cp\u003eDetection\u003cbr\u003e\nrange: 246.9-20,000pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 91.3pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eAssay Time: 1h, 10min\u003c\/p\u003e\n\n\u003cp\u003eSample Size: 100uL\u003c\/p\u003e\n\n\u003cp\u003eRecommended\/Predicted\u003cbr\u003e\nSample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Precision: Intra-Assay: CV\u0026lt;10%, Inter-Assay: CV\u0026lt;12%\u003c\/p\u003e\n\n\u003cp\u003eStorage: 4°C for 1 month\/ -20°C for long-term(One year within shelf life)\u003c\/p\u003e\n\n\u003cp\u003eShelf-life: 12 months\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This assay has high sensitivity and excellent specificity for detection of Instant Procollagen I N-Terminal Propeptide (PINP).\u003cbr\u003e\nNo significant cross-reactivity or interference between Instant Procollagen I N-Terminal Propeptide (PINP) and analogues was observed.\u003c\/p\u003e\n\n\u003cp\u003ePrecision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Procollagen I N-Terminal Propeptide (PINP) were tested 20 times on one plate, respectively.\u003cbr\u003e\nInter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Procollagen I N-Terminal Propeptide (PINP) were tested on 3 different plates, 8 replicates in each plate.\u003cbr\u003e\nCV(%) = SD\/meanX100\u003c\/p\u003e\n\n\u003cp\u003eStability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.\u003cbr\u003e\nTo minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\u003c\/p\u003e\n\n\u003cp\u003eTarget: 1. Prepare all reagents, samples and standards;\u003cbr\u003e\n2. Add 50µL standard or sample to each well.\u003cbr\u003e\nAnd then add 50µL prepared Detection Reagent A immediately.\u003cbr\u003e\nShare and mix. Incubate 50 minutes at 37°C;\u003cbr\u003e\n3. Aspirate and wash 3 times;\u003cbr\u003e\n4. Add 100µL prepared Detection Reagent B. Incubate 10 minutes at 37°C;\u003cbr\u003e\n5. Aspirate and wash 5 times;\u003cbr\u003e\n6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;\u003cbr\u003e\n7. Add 50µL Stop Solution. Read at 450nm immediately.\u003c\/p\u003e\n\n\u003cp\u003eTest principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Instant Procollagen I N-Terminal Propeptide (PINP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Instant Procollagen I N-Terminal Propeptide (PINP) and unlabeled Instant Procollagen I N-Terminal Propeptide (PINP) (Standards or samples) with the pre-coated antibody specific to Instant Procollagen I N-Terminal Propeptide (PINP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Instant Procollagen I N-Terminal Propeptide (PINP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Instant Procollagen I N-Terminal Propeptide (PINP) in the sample.\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Metabolic pathway; Hepatology; Bone metabolism;\u003c\/p\u003e \n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Cloud-Clone","offers":[{"title":"24T","offer_id":45379796074675,"sku":"IEA957Ra-24","price":574.0,"currency_code":"CAD","in_stock":true},{"title":"48T","offer_id":45379796107443,"sku":"IEA957Ra-48","price":1005.2,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45379796140211,"sku":"IEA957Ra-96","price":1436.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Cloud-Clone.jpg?v=1774117629","url":"https:\/\/afsbio.com\/products\/instant-elisa-kit-for-procollagen-i-n-terminal-propeptide-pinp-iea957ra","provider":"AFSBio Inc.","version":"1.0","type":"link"}