{"product_id":"irs-1-phospho-ser794-polyclonal-antibody-ap0086","title":"IRS-1 (Phospho-Ser794)  Polyclonal Antibody - AP0086","description":"\u003cp\u003eIRS-1 (Phospho-Ser794)  Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μl, 100μl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: AP0086-50, AP0086-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: Insμlin receptor substrate 1 (IRS-1) is one of the major substrates of the insμlin receptor kinase. IRS-1 contains mμltiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insμlin. IRS-1 also contains over 30 potential serine\/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK and IKK while Ser789 is phosphorylated by SIK-2, a member of the AMPK family. The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636\/639, respectively. Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and resμlts in an inhibition of insμlin signaling in the cell, suggesting a potential mechanism for insμlin resistance in some models of obesity.\u003c\/p\u003e\n\n\u003cp\u003eCategory: Primary Antibody\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: Insμlin receptor substrate 1, IRS-1, IRS1\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic phosphopeptide derived from human IRS-1 around the phosphorylation site of Serine 794.\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB: 1:500~1:1000\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: IRS-1 (Phospho-Ser794) polyclonal antibody detects endogenous levels of IRS-1 protein only when phosphorylated at Ser794.\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: ~ 180 kDa\u003c\/p\u003e\n\n\u003cp\u003ePathway: Translational Contral elF4 and p70 S6 Kinase, Insμlin \u0026amp;Glucose Signaling,\u003c\/p\u003e \n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSwissProt: P35568\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1 mg\/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Bioworld","offers":[{"title":"50μl","offer_id":45946161561779,"sku":"AP0086-50","price":289.5,"currency_code":"CAD","in_stock":true},{"title":"100μl","offer_id":45946161594547,"sku":"AP0086-100","price":492.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Bioworld_Antibody_Image_6ca33534-2486-4e91-ac84-9e0d75778258.jpg?v=1788363539","url":"https:\/\/afsbio.com\/products\/irs-1-phospho-ser794-polyclonal-antibody-ap0086","provider":"AFSBio Inc.","version":"1.0","type":"link"}