{"product_id":"isy1-polyclonal-antibody-bs72657","title":"ISY1 Polyclonal Antibody-BS72657","description":"\u003cp\u003eISY1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS72657-50, BS72657-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9ULR0\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Component of the spliceosome C complex required for the selective processing of microRNAs during embryonic stem cell differentiation (By similarity. Required for the biogenesis of all miRNAs from the pri-miR-17-92 primary transcript except miR-92a (By similarity. Only required for the biogenesis of miR-290 and miR-96 from the pri-miR-290-295 and pri-miR-96-183 primary transcripts, respectively (By similarity. Required during the transition of embryonic stem cells (ESCs from the naive to primed state (By similarity. By enhancing miRNA biogenesis, promotes exit of ESCs from the naive state to an intermediate state of poised pluripotency, which precedes transition to the primed state (By similarity. Involved in pre-mRNA splicing as component of the spliceosome.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Polyclonal Antibodies\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 38kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western Blot analysis of extracts of various cell lines, using ISY1 antibody at 1:3000 dilution.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Basic Kit.\u003cbr\u003e\nExposure time: 90s.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: ISY1; FSAP33\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human ISY1(NP_001186398.1).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42927655518387,"sku":"BS72657-50","price":334.88,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42927655551155,"sku":"BS72657-100","price":576.38,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_342e500c-8541-490e-a230-d2d7429fc53b.jpg?v=1704109669","url":"https:\/\/afsbio.com\/products\/isy1-polyclonal-antibody-bs72657","provider":"AFSBio Inc.","version":"1.0","type":"link"}