{"product_id":"jnk1-2-3-phospho-t183-rabbit-monoclonal-antibody-bs9939m","title":"JNK1\/2\/3 (Phospho-T183) Rabbit Monoclonal Antibody -BS9939M","description":"\u003cp\u003eJNK1\/2\/3 (Phospho-T183) Rabbit Monoclonal Antibody\u003c\/p\u003e \n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS9939M-50, BS9939M-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P45983\/P45984\/P53779\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\/IF, IHC, FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:1000-1:2000 \u003cbr\u003e\nIHC\/ICC\/IF: 1:50-1:200 \u003cbr\u003e\nFC: 1:50-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. Capsaicin activates JNK1 and p38 in Ras-transformed human breast epithelial cells. Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos, c-Jun and other signaling kinases, including MEKK1 and p38. JNK3 (MK10, MAPK10, PRKM10) is activated by pro-inflammatory cytokines and environmental stresss by phosphorylating transcription factors such as c-Jun and ATF2. This is important for AP-1 transcriptional activity regulation. JNK3 is crucial for neuronal apoptosis (stress-induced).\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Protein A affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects endogenous levels of JNK1 protein only when phosphorylated at Thr183. This antibody also recognizes JNK2 and JNK3 when phosphorylated at the corresponding residues.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 46, 54 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of JNK1\/2\/3 (Phospho-T183) Rabbit mAb at 1:1000 dilution\u003cbr\u003e\n Lane 1: NIH\/3T3 whole cell lysate treated with Anisomycin\u003cbr\u003e\n Lane 2: NIH\/3T3 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Phospho-JNK1\/2\/3(T183) antibody. Counter stained with hematoxylin., ICC staining Phospho-JNK1\/2\/3(T183) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: JNK 46; JNK 55; JNK; JNK1; JNK1A2; JNK2; JNK21B1\/2; JNK2ALPHA; JNK2B; JNK2BETA; JNK3; JNK3A; MAP kinase 8; MAP kinase 9; MAP kinase p49 3F12; MAPK 8; MAPK 9; MAPK10; MAPK8; MAPK9; MGC50974; Mitogen activated protein kinase 10; Mitogen activated protein kinase 8; Mitogen activated protein kinase 9;\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: Recombinant antibody.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43044034871475,"sku":"BS9939M-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43044034904243,"sku":"BS9939M-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_e703d84d-d333-49b2-8fd6-8c1aa4eab1f1.jpg?v=1707738807","url":"https:\/\/afsbio.com\/products\/jnk1-2-3-phospho-t183-rabbit-monoclonal-antibody-bs9939m","provider":"AFSBio Inc.","version":"1.0","type":"link"}