1x 500 µl Protein Marker in loading buffer, sufficient for 100 mini gels or 50 large gels
Broad range: 10 – 180 kDa
Catalogue Number: MWP05
JustBlue Prestained Protein Marker
The JustBlue Protein Marker is a reasonable priced alternative to the BlueStar Marker. Eleven proteins are coupled with a blue chromophore. This Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon or nitrocellulose) and for approximating the size of proteins. We recommend 4-5 µl per well for protein electrophoresis and 2-3 µl per well for Western Transfer.
- Broad range: 10 – 180 kDa
- High performance: 11 bands with 1 colour
- Ready to use: Supplied in a loading buffer for direct loading on gels.
- Reference bands: 28 kDa and 72 kDa with higher intensity
- Sharp bands
Stable at -20°C for 12 months.
Stable at +4°C for 3 months.
- Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis
- Monitoring of protein transfer onto membranes during Western blotting
- Sizing of proteins on SDS-polyacrylamide gels and Western blots
Could you send me information about the concentration of each individual band of the JustBlue Prestained Protein Marker. This because someone wants to use it to estimate protein amount of his samples. The only info I have is that the concentration is 0.1 to 0.5 mg/ml. Does that mean 0.1mg/ml for normal bands and 0.5mg/ml for the 25kDa and 75kDa bands?
Unfortunately, in general it is not possible to use dyed protein standards in order to estimate the amount of protein sample. Since the dyes used to couple with the proteins of the ladder are different from the Coomassie Blue dye used to stain the protein(s) of interest, it’d help your customer to obtain a more accurate molecular weight estimation by using an unstained protein ladder because both the unstained protein ladder and the target protein(s) are then stained with the Coomassie Blue dye.
Does this marker contain Formaldehyde or Formamide?