{"product_id":"kaiso-zbtb33-polyclonal-antibody-bs72232","title":"Kaiso\/ZBTB33 Polyclonal Antibody-BS72232","description":"\u003cp\u003eKaiso\/ZBTB33 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50 µl, 100 µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS72232-50, BS72232-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q86T24\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eKaiso\/ZBTB33 Polyclonal Antibody-BS72232\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ChIP,\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:1000 - 1:2000  |  ChIP,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: This gene encodes a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ\/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway, and may also activate transcription of a subset of target genes by the recruitment of catenin delta-2 (CTNND2). Its interaction with catenin delta-1 (CTNND1) inhibits binding to both methylated and non-methylated DNA. It also interacts directly with the nuclear import receptor Importin-alpha2 (also known as karyopherin alpha2 or RAG cohort 1), which may mediate nuclear import of this protein. Alternatively spliced transcript variants encoding the same protein have been identified.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Polyclonal Antibodies\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 74-90kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using Kaiso\/Kaiso\/ZBTB33 antibody at 1:3000 dilution.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Enhanced Kit.\u003cbr\u003e\nExposure time: 90s., Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Kaiso\/ZBTB33 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: ZBTB33; ZNF-kaiso; ZNF348\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human Kaiso\/Kaiso\/ZBTB33(NP_006768.1).\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42962862276787,"sku":"BS72232-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42962862309555,"sku":"BS72232-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_74751b16-0d73-4d95-9375-0ae9b49a08e5.jpg?v=1705319414","url":"https:\/\/afsbio.com\/products\/kaiso-zbtb33-polyclonal-antibody-bs72232","provider":"AFSBio Inc.","version":"1.0","type":"link"}