{"product_id":"kdel-polyclonal-antibody-bs90764","title":"KDEL Polyclonal Antibody-BS90764","description":"\u003cp\u003eKDEL Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90764-50, BS90764-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P24390(Human) Q99JH8(Mouse) Q569A6(Rat)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC, IF, IHC, FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500-1:2000\u003cbr\u003e\nICC:1:400-1:800\u003cbr\u003e\nIHC:1:100-1:400\u003cbr\u003e\nFC:1:50-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: Soluble proteins in the endoplasmic reticulum (ER) contain a specific carboxy terminal sequence KDEL (Lys-Asp-Glu-Leu), and include the coat proteins required for vesicle budding from the ER, proteins that form retrograde vesicles on post-ER compartments, and integral membrane proteins that target vesicles to their correct destination. The retention of these soluble proteins in the ER depends on the interaction of the KDEL sequence with the corresponding KDEL receptor, also designated ERD2, in the Golgi apparatus. When KDEL proteins reach the Golgi complex, they are recognized by the KDEL receptor and transported retrograde in COPI-coated vesicles back to the ER. The small GTPase ADP-ribosylation factor 1 (ARF1), a regulator of vesicle transport, interacts with the KDEL receptor. Subsequently, this interaction allows the KDEL receptor to recruit a GTPase-activating protein (GAP) from the cytosol to membranes, which inactivates ARF1.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProA affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: KDEL polyclonal antibody detects endogenous levels of KDEL protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 24\/17 kDa(Predicted band size)\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of KDEL on different lysates using anti-KDEL antibody at 1\/1,000 dilution.\u003cbr\u003e\n Positive control:\u003cbr\u003e\n Lane 1: Rat testis tissue\u003cbr\u003e\n Lane 2: Human placenta tissue\u003cbr\u003e\n Lane 3: Mouse testis tissue\u003cbr\u003e\n Lane 4: 293, ICC staining KDEL in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: KDEL receptor 1, KDELR1, ERD2.1, ER lumen protein retaining receptor 1, ERD2.1, ERD21, KDEL endoplasmic reticulum protein retention receptor 1, KDEL receptor 1, Kdelr1, Putative MAPK-activating protein PM23,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: Recombinant protein\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42984585363635,"sku":"BS90764-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42984585396403,"sku":"BS90764-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_6b7ccfdd-e6c3-4882-a11e-8899de35cf91.jpg?v=1705918758","url":"https:\/\/afsbio.com\/products\/kdel-polyclonal-antibody-bs90764","provider":"AFSBio Inc.","version":"1.0","type":"link"}