{"product_id":"kdm5a-polyclonal-antibody-bs78845","title":"KDM5A Polyclonal Antibody-BS78845","description":"\u003cp\u003eKDM5A Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS78845-50, BS78845-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P29375\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP, , ChIP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000   |   IP,1:50 - 1:200   |   ChIP,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Unmodification\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: 192kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using KDM5A antibody at 1:500 dilution.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\n Detection: ECL Enhanced Kit.\u003cbr\u003e\n Exposure time: 60s., Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug KDM5A antibody. Western blot was performed from the immunoprecipitate using KDM5A antibody at a dilution of 1:500., Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using KDM5A antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: KDM5A; RBBP-2; RBBP2; RBP2\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human KDM5A.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42997531836595,"sku":"BS78845-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42997531869363,"sku":"BS78845-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_b9e23628-8960-45b6-b97e-2c35d985beee.jpg?v=1706270103","url":"https:\/\/afsbio.com\/products\/kdm5a-polyclonal-antibody-bs78845","provider":"AFSBio Inc.","version":"1.0","type":"link"}