{"product_id":"kif2c-phospho-s95-polyclonal-antibody-bs67093","title":"KIF2C (phospho-S95) Polyclonal Antibody-BS67093","description":"\u003cp\u003eKIF2C (phospho-S95) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS67093-50, BS67093-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q99661\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB (1\/500 - 1\/1000), IF\/IC (1\/100 - 1\/500)\u003c\/p\u003e\n\n\u003cp\u003eBackground: In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells (PubMed:21820309).Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis (PubMed:19060894).Plays a role in chromosome congression and is required for the lateral to end-on conversion of the chromosome-microtubule attachment (PubMed:23891108).\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of KIF2C (pS95) protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 90 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of KIF2C (pS95) expression in H1688 (A), H446 (B) whole cell lysates., Immunofluorescent analysis of KIF2C (pS95) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: KNSL6; Kinesin-like protein KIF2C; Kinesin-like protein 6; Mitotic centromere-associated kinesin; MCAK\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human KIF2C. The exact sequence is proprietary.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42989557153971,"sku":"BS67093-50","price":276.71,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42989557186739,"sku":"BS67093-100","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_88a5c17c-51de-4dbb-8538-aed3746f8e4f.jpg?v=1706095368","url":"https:\/\/afsbio.com\/products\/kif2c-phospho-s95-polyclonal-antibody-bs67093","provider":"AFSBio Inc.","version":"1.0","type":"link"}