{"product_id":"klhdc2-polyclonal-antibody-bs79954","title":"KLHDC2 Polyclonal Antibody-BS79954","description":"\u003cp\u003eKLHDC2 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS79954-50, BS79954-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9Y2U9\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Substrate-recognition component of a Cul2-RING (CRL2 E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms. The CRL2(KLHDC2 complex specifically recognizes proteins with a diglycine (Gly-Gly at the C-terminus, leading to their ubiquitination and degradation. The CRL2(KLHDC2 complex mediates ubiquitination and degradation of truncated SELENOK and SELENOS selenoproteins produced by failed UGA\/Sec decoding, which end with a diglycine. The CRL2(KLHDC2 complex also recognizes proteolytically cleaved proteins ending with Gly-Gly, such as the N-terminal fragment of USP1, leading to their degradation. May also act as an indirect repressor of CREB3-mediated transcription by interfering with CREB3-DNA-binding.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Unmodification\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: 46kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of K-562 cells, using KLHDC2 antibody at 1:1000 dilution.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Basic Kit.\u003cbr\u003e\nExposure time: 150s.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: KLHDC2; HCLP-1; HCLP1; LCP\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human KLHDC2(NP_055130.1).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42946873917619,"sku":"BS79954-50","price":334.88,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42946873983155,"sku":"BS79954-100","price":576.38,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_72000288-1028-4362-bcf3-58445b56efc6.jpg?v=1704884218","url":"https:\/\/afsbio.com\/products\/klhdc2-polyclonal-antibody-bs79954","provider":"AFSBio Inc.","version":"1.0","type":"link"}