{"product_id":"ko-validated-cdk4-rabbit-polymab®-a23521pm","title":"[KO Validated] CDK4 Rabbit PolymAb® - A23521PM","description":"\u003cp\u003e[KO Validated] CDK4 Rabbit PolymAb®\u003c\/p\u003e\n\n\u003cp\u003eSizes: 20μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: A23521PM-20, A23521PM-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: The protein encoded by this gene is a member of the Ser\/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16 (INK4a) . This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb) . Mutations in this gene as well as in its related proteins including D-type cyclins, p16 (INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.\u003c\/p\u003e\n\n\u003cp\u003eCategory: Monoclonal Antibodies\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP, IF\/ICC, IF-P, IHC-P, ELISA\u003c\/p\u003e\n\n\u003cp\u003eCross-reactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eProtein Weight: 34kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved Molecular Weight: 34kDa\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide .\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 1019\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P11802\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: CMM3; PSK-J3\u003c\/p\u003e\n\n\u003cp\u003eSource: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003ePurity: Affinity purification\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20℃. Avoid freeze \/ thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.\u003c\/p\u003e\n\n\u003cp\u003eRecommended Dilutions: WB, 1:1000 - 1:12000 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells IF\/ICC, 1:200 - 1:800 IF-P, 1:200 - 1:800 IHC-P, 1:200 - 1:800 ELISA, Recommended starting concentration is 1 μg\/mL. Please optimize the concentration based on your specific assay requirements.\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Epigenetics Nuclear Signaling, Protein phosphorylation, Cancer, Signal Transduction, Kinase, Serine threonine kinases, Cell Biology Developmental Biology, Cell Cycle, Cell Cycle Control-G1 S Checkpoint.\u003c\/p\u003e\n\n\u003cp\u003eNCBI Alias: CDK4\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"20uL","offer_id":45189843746995,"sku":"A23521PM-20","price":165.2,"currency_code":"CAD","in_stock":true},{"title":"100uL","offer_id":45189843779763,"sku":"A23521PM-100","price":431.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_f18968c3-aebe-4c9d-8c46-1435043581d3.png?v=1766436586","url":"https:\/\/afsbio.com\/products\/ko-validated-cdk4-rabbit-polymab%c2%ae-a23521pm","provider":"AFSBio Inc.","version":"1.0","type":"link"}