{"product_id":"ko-validated-gcn1l1-polyclonal-antibody-bs72185","title":"[KO Validated] GCN1L1 Polyclonal Antibody-BS72185","description":"\u003cp\u003e[KO Validated] GCN1L1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50 µl, 100 µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS72185-50, BS72185-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q92616\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Acts as a positive activator of the EIF2AK4\/GCN2 protein kinase activity in response to amino acid starvation. Forms a complex with EIF2AK4\/GCN2 on translating ribosomes; during this process, GCN1 seems to act as a chaperone to facilitate delivery of uncharged tRNAs that enter the A site of ribosomes to the tRNA-binding domain of EIF2AK4\/GCN2, and hence stimulating EIF2AK4\/GCN2 kinase activity. Participates in the repression of global protein synthesis and in gene-specific mRNA translation activation, such as the transcriptional activator ATF4, by promoting the EIF2AK4\/GCN2-mediated phosphorylation of eukaryotic translation initiation factor 2 (eIF-2-alpha\/EIF2S1 on 'Ser-52', and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts from wild type and GCN1L1 knockout 293T cells, using GCN1L1 antibody at 1:3000 dilution. \u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. \u003cbr\u003e\nLysates\/proteins: 25ug per lane. \u003cbr\u003e\nBlocking buffer: 3% non-fat dry milk in TBST. \u003cbr\u003e\nDetection: ECL Basic Kit. \u003cbr\u003e\nExposure time: 30s.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 293KDa\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Polyclonal Antibodies\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: GCN1; GCN1L; GCN1L1; PRIC295\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human GCN1L1(NP_006827.1).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42939211808947,"sku":"BS72185-50","price":415.38,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42939211841715,"sku":"BS72185-100","price":640.78,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_c785eb6f-513b-4e1b-8d6d-4b4e8baa6ac3.jpg?v=1704545187","url":"https:\/\/afsbio.com\/products\/ko-validated-gcn1l1-polyclonal-antibody-bs72185","provider":"AFSBio Inc.","version":"1.0","type":"link"}