{"product_id":"ko-validated-gcn1l1-polyclonal-antibody-e-ab-64442","title":"(KO Validated) GCN1L1 Polyclonal Antibody - E-AB-64442","description":"\u003cp\u003e(KO Validated) GCN1L1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 60μL, 120μL, 200μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-AB-64442-60, E-AB-64442-120, E-AB-64442-200\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: GCN1L1\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: GCN1; GCN1L; GCN1L1; PRIC295\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Cancer,Epigenetics and Nuclear Signaling\u003c\/p\u003e\n\n\u003cp\u003eConjugation: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eSpecies Reactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplication: WB\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n \n\n\u003cp\u003eUNIProt ID: Q92616\u003c\/p\u003e\n\n\u003cp\u003eBackground: Acts as a positive activator of the EIF2AK4\/GCN2 protein kinase activity in response to amino acid starvation. Forms a complex with EIF2AK4\/GCN2 on translating ribosomes; during this process, GCN1 seems to act as a chaperone to facilitate delivery of uncharged tRNAs that enter the A site of ribosomes to the tRNA-binding domain of EIF2AK4\/GCN2, and hence stimulating EIF2AK4\/GCN2 kinase activity. Participates in the repression of global protein synthesis and in gene-specific mRNA translation activation, such as the transcriptional activator ATF4, by promoting the EIF2AK4\/GCN2-mediated phosphorylation of eukaryotic translation initiation factor 2 (eIF-2-alpha\/EIF2S1 on 'Ser-52', and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/mL\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human GCN1L1\u003c\/p\u003e\n\n\u003cp\u003eBuffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3.\u003c\/p\u003e\n\n\u003cp\u003ePurification Method: Affinity purification\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB 1:500-1:2000\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 292kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 293KDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: -20°C\/One year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: Ice bag\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"60μL","offer_id":43146329882803,"sku":"E-AB-64442-60","price":308.0,"currency_code":"CAD","in_stock":true},{"title":"120μL","offer_id":43146329915571,"sku":"E-AB-64442-120","price":504.0,"currency_code":"CAD","in_stock":true},{"title":"200μL","offer_id":43146329948339,"sku":"E-AB-64442-200","price":812.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_9e1ed19b-9610-4920-b2ff-a50aa0dfb936.png?v=1710293720","url":"https:\/\/afsbio.com\/products\/ko-validated-gcn1l1-polyclonal-antibody-e-ab-64442","provider":"AFSBio Inc.","version":"1.0","type":"link"}