{"product_id":"ko-validated-hnrnp-e1-pcbp1-polyclonal-antibody-bs78254","title":"[KO Validated] hnRNP E1\/PCBP1 Polyclonal Antibody-BS78254","description":"[KO Validated] hnRNP E1\/PCBP1 Polyclonal Antibody\n\nSizes: 50µl, 100µl\n\nCatalogue Numbers: BS78254-50, BS78254-100\n\nProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\n\nSwiss-Prot: Q15365\n\nHost: Rabbit\n\nReactivity: Human, Mouse, Rat\n\nApplications: WB, IHC\n\nAll Applications: WB,1:500 - 1:2000   |   IHC,1:50 - 1:200\n\nBackground: This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.\n\nPurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\n\nStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\n\nSpecificity: Unmodification \n\nBioworld Molecular Weight: 40KDa\n\nNote: For research use only, not for use in diagnostic procedure.\n\nExtra Notes: Western blot analysis of extracts of various cell lines, using hnRNP E1\/PCBP1 antibody at 1:1000 dilution.\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\n Lysates\/proteins: 25ug per Lane.\n Blocking buffer: 3% non-fat dry milk in TBST.\n Detection: ECL Basic Kit.\n Exposure time: 30s., Western blot analysis of extracts from wild type and hnRNP E1\/PCBP1 knockout HeLa cells, using hnRNP E1\/PCBP1 antibody at 1:1000 dilution.\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\n Lysates\/proteins: 25ug per Lane.\n Blocking buffer: 3% non-fat dry milk in TBST.\n Detection: ECL Basic Kit.\n Exposure time: 30s., Immunohistochemistry of paraffin-embedded human esophageal cancer using hnRNP E1\/PCBP1 Rabbit pAb at dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse testis using hnRNP E1\/PCBP1 Rabbit pAb at dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.\n\nAlternative Name: PCBP1; HEL-S-85; HNRPE1; HNRPX; hnRNP-E1; hnRNP-X; poly(rC)-binding protein 1\n\nImmunogen: A synthetic peptide of human hnRNP E1\/PCBP1(NP_006187.2).\n\nConjugate: Unconjugated\n\nModification: Unmodified ","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43058767691955,"sku":"BS78254-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43058767724723,"sku":"BS78254-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_a4cfb6a9-acc2-472b-b32b-6b4f2917fd03.jpg?v=1708171989","url":"https:\/\/afsbio.com\/products\/ko-validated-hnrnp-e1-pcbp1-polyclonal-antibody-bs78254","provider":"AFSBio Inc.","version":"1.0","type":"link"}