{"product_id":"ko-validated-rag2-polyclonal-antibody-bs65018","title":"[KO Validated] RAG2 Polyclonal Antibody-BS65018","description":"\u003cp\u003e[KO Validated] RAG2 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS65018-50, BS65018-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P55895\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB\t1:500 - 1:2000  IF\t1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: RAG2 polyclonal antibody detects endogenous levels of RAG2 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 59 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using RAG2 antibody at 1:1000 dilution., Western blot analysis of extracts from normal (control) and RAG2 Rabbit pAb knockout (KO) 293T cells, using RAG2 Rabbit pAb antibody at 1:500 dilution., Immunofluorescence analysis of U2OS cells using RAG2 antibody. Blue: DAPI for nuclear staining., Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using RAG2 antibody. Green：GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: RAG2; RAG-2\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 258-527 of human RAG2\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43050226221235,"sku":"BS65018-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43050226254003,"sku":"BS65018-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_b660f6af-2001-4333-8fc5-99b18fc9f3c7.jpg?v=1707913621","url":"https:\/\/afsbio.com\/products\/ko-validated-rag2-polyclonal-antibody-bs65018","provider":"AFSBio Inc.","version":"1.0","type":"link"}