{"product_id":"ksr-1-polyclonal-antibody-ra26730","title":"Ksr-1 Polyclonal Antibody - RA26730","description":"\u003cp\u003eKsr-1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RA26730-50, RA26730-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,function:Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.,PTM:Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated.,similarity:Belongs to the protein kinase superfamily. TKL Ser\/Thr protein kinase family.,similarity:Contains 1 phorbol-ester\/DAG-type zinc finger.,similarity:Contains 1 protein kinase domain.,subcellular location:In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery.,subunit:Interacts with HSPCA\/HSP90, YWHAB\/14-3-3, CDC37, MAP2K\/MEK, MARK3, PPP2R1A and PPP2CA. Also interacts with RAF and MAPK\/ERK, in a Ras-dependent manner (By similarity) . The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization.,\u003c\/p\u003e\n\n\u003cp\u003eCondition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eStorage Instructions: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eRecommended dilutions: Western Blot: 1\/500 - 1\/2000. Immunohistochemistry: 1\/100 - 1\/300. Immunofluorescence: 1\/200 - 1\/1000. ELISA: 1\/20000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: KSR1; KSR; Kinase suppressor of Ras 1\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB; IHC-p; IF\/ICC; ELISA\u003c\/p\u003e\n\n\u003cp\u003eSpecies Cross-Reactivity: Human; Mouse\u003c\/p\u003e\n\n\u003cp\u003eGeneID (Human): 8844\u003c\/p\u003e\n\n\u003cp\u003eProtein MW (KDa): 115\u003c\/p\u003e\n\n\u003cp\u003eSWISS: Q8IVT5\u003c\/p\u003e\n\n\u003cp\u003eSource: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Reed Bio","offers":[{"title":"50μL","offer_id":45121373012147,"sku":"RA26730-50","price":336.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":45121373044915,"sku":"RA26730-100","price":448.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/logo_4ecc8b47-ac63-482e-8b28-6834ac24fef5.png?v=1773346927","url":"https:\/\/afsbio.com\/products\/ksr-1-polyclonal-antibody-ra26730","provider":"AFSBio Inc.","version":"1.0","type":"link"}