{"product_id":"ksr1-polyclonal-antibody-bs76133","title":"KSR1 Polyclonal Antibody-BS76133","description":"\u003cp\u003eKSR1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS76133-50, BS76133-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q8IVT5\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eKSR1 Polyclonal Antibody-BS76133\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000  |  IF\/ICC,1:50 - 1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: Part of a multiprotein signaling complex which promotes phosphorylation of Raf family members and activation of downstream MAP kinases (By similarity. Independently of its kinase activity, acts as MAP2K1\/MEK1 and MAP2K2\/MEK2-dependent allosteric activator of BRAF; upon binding to MAP2K1\/MEK1 or MAP2K2\/MEK2, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1\/MEK1 and\/or MAP2K2\/MEK2. Promotes activation of MAPK1 and\/or MAPK3, both in response to EGF and to cAMP (By similarity. Its kinase activity is unsure (By similarity. Some protein kinase activity has been detected in vitro, however the physiological relevance of this activity is unknown (By similarity.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Unmodification\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 110kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using KSR1 antibody at 1:1000 dilution.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Basic Kit.\u003cbr\u003e\nExposure time: 10s., Immunofluorescence analysis of C6 cells using KSR1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: KSR1; KSR; RSU2\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human KSR1(NP_055053.1).\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42960040591539,"sku":"BS76133-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42960040624307,"sku":"BS76133-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_481cd2a7-62d8-48f0-8064-c8cf53c1a9c8.jpg?v=1705230864","url":"https:\/\/afsbio.com\/products\/ksr1-polyclonal-antibody-bs76133","provider":"AFSBio Inc.","version":"1.0","type":"link"}