{"product_id":"laminin-r-q215-polyclonal-antibody-bs3689","title":"Laminin-R (Q215) Polyclonal Antibody-BS3689","description":"\u003cp\u003eLaminin-R (Q215) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS3689-50, BS3689-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P08865\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003cbr\u003e\nIF: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Laminin receptor (Laminin-R) has a heterodimeric structure similar to that of receptors for other extracellular matrix proteins such as Fibronectin and Vitronectin. Incorporation of Laminin-R into lysosomal membranes makes it possible for lysosomes to attach to surfaces coated with Laminin. This and other properties identify Laminin-R as a member of the integrin family of cell adhesion receptors. The Laminin-R precursor is a polypeptide whose expression is consistently upregulated in aggressive carcinoma. Laminin-R (also known as colon carcinoma laminin-binding protein) and is found at nine-fold higher levels in colon carcinoma than in adjacent normal colonic epithelium. Additionally, the level of the Laminin-R is higher in the lung cancer cell line than in the lung cell line.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Laminin-R (Q215) polyclonal antibody detects endogenous levels of Laminin-R protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 40 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Laminin-R (Q215) polyclonal antibody at 1:500 dilution\u003cbr\u003e\n Lane 1: CT26 whole cell lysate (40ug)\u003cbr\u003e\n Lane 2: PC12 whole cell lysate (40ug)\u003cbr\u003e\n Lane 3: A549 whole cell lysate (40ug)\u003cbr\u003e\n Lane 4: HEK293T whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Laminin-R (Q215) pAb in paraffin-embedded human breast carcinoma tissue at 1:50., IF image of BS3689 stained A549 cells. The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody Laminin-R (Q215) #BS3689(1:100) at 10µg\/ml overnight at +4°C. The secondary antibody (Green) was Goat Anti-Rabbit IgG (H+L) FITC #BS10950 used at a 1\/1000 dilution for 1h. Hoechst33342 #BD5011 was used to stain the cell nuclei (blue).\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: 40S ribosomal protein SA; 37 kDa laminin receptor precursor; 37LRP; 37\/67 kDa laminin receptor; LRP\/LR; 67 kDa laminin receptor; 67LR; Colon carcinoma laminin-binding protein; Laminin receptor 1; LamR; Laminin-binding protein precursor p40; LBP\/p40; Multidrug resistance-associated protein MGr1-Ag; NEM\/1CHD4; LAMBR; LAMR1; RPSA\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 181-230 of Human Laminin-R.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42993798348979,"sku":"BS3689-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42993798381747,"sku":"BS3689-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_c088a0ad-79ee-4126-ace4-a29da5893dcf.jpg?v=1706183681","url":"https:\/\/afsbio.com\/products\/laminin-r-q215-polyclonal-antibody-bs3689","provider":"AFSBio Inc.","version":"1.0","type":"link"}