{"product_id":"lc3a-8f5-monoclonal-antibody-mb3283","title":"LC3A (8F5) Monoclonal Antibody-MB3283","description":"\u003cp\u003eLC3A (8F5) Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB3283-50, MB3283-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9H492\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Rat, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC-P\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1\/500-1\/1000  IHC: 1\/50-1\/100\u003c\/p\u003e  \n\n\u003cp\u003eBackground: Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3), and subsequently found to contain similarity to the yeast protein Apg8\/Aut7\/Cvt5 critical for autophagy. Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy. Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Affinity Purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: IgG1\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: Calculated MW: 14 kDa; Observed MW: 14,16 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of LC3A in Hela lysates, 3T3 lysates, rat Brain lysates using LC3A antibody., Immunohistochemistry analysis of paraffin-embedded Human Heptacarcinoma Tissue using LC3A antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunohistochemistry analysis of paraffin-embedded Human Prostate Carcinoma Tissue using LC3A antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Microtubule-associated proteins 1A\/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A\/MAP1B light chain 3 A; MAP1A\/MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alpha\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Purified recombinant protein expressed in E.coli.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42999931240627,"sku":"MB3283-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42999931273395,"sku":"MB3283-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_379eb932-dd90-4da7-98e2-86c75838650f.jpg?v=1706358251","url":"https:\/\/afsbio.com\/products\/lc3a-8f5-monoclonal-antibody-mb3283","provider":"AFSBio Inc.","version":"1.0","type":"link"}