{"product_id":"lipolysis-colorimetric-assay-kit-e-bc-k798-m","title":"Lipolysis Colorimetric Assay Kit - E-BC-K798-M","description":"\u003cp\u003eLipolysis Colorimetric Assay Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-BC-K798-M-48, E-BC-K798-M-96\u003c\/p\u003e\n\n\u003cp\u003eLead times: 2-3 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: Lipolysis is the process by which the body converts fat reserves into energy, the fat stored in fat cells is gradually hydrolyzed under the catalysis of lipase, releasing free fatty acid (FFA) and glycerin (Gly). The glycerol produced by lipolysis cannot be used by the cells themselves, and needs to be transported to the liver for further conversion into energy. The content of glycerol can reflect the level of lipolysis in the cell sample.\u003cbr\u003e\nThe detection principle of this kit is to make the glycerol released by cells produce hydrogen peroxide under the catalysis of lipase, and hydrogen peroxide reacts with enzymes and chemical substances to produce specific products, which have maximum absorption at 555 nm, and OD value is proportional to the concentration within a certain range.\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Glycolysis and Lipid Metabolism;Metabolic Diseases\u003c\/p\u003e\n\n\u003cp\u003eResult Type: Quantitative\u003c\/p\u003e\n\n\u003cp\u003eDetection Instrument: Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eDetection Type: Colorimetric method\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.005-0.543 mmol\/L\u003c\/p\u003e\n\n\u003cp\u003eTested Sample Types: Cell\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 30 min\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.005 mmol\/L\u003c\/p\u003e\n\n\u003cp\u003eInter cv: 1.6-2.5%\u003c\/p\u003e\n\n\u003cp\u003eIntra cv: 1.3-1.4%\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"48T","offer_id":46264647712947,"sku":"E-BC-K798-M-48","price":478.5,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":46264647745715,"sku":"E-BC-K798-M-96","price":693.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/Screenshot_2024-02-12_153513_49a86b0b-f422-42b0-baac-7435bb35d3d6.png?v=1707826183","url":"https:\/\/afsbio.com\/products\/lipolysis-colorimetric-assay-kit-e-bc-k798-m","provider":"AFSBio Inc.","version":"1.0","type":"link"}