{"product_id":"lrpprc-polyclonal-antibody-bs70542","title":"LRPPRC Polyclonal Antibody-BS70542","description":"\u003cp\u003eLRPPRC Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS70542-50, BS70542-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P42704\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:1000 - 1:2000   |   IHC,1:100 - 1:200   |   IF\/ICC,1:100 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: This gene encodes a leucine-rich protein that has multiple pentatricopeptide repeats (PPR). The precise role of this protein is unknown but studies suggest it may play a role in cytoskeletal organization, vesicular transport, or in transcriptional regulation of both nuclear and mitochondrial genes. The protein localizes primarily to mitochondria and is predicted to have an N-terminal mitochondrial targeting sequence. Mutations in this gene are associated with the French-Canadian type of Leigh syndrome.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Polyclonal Antibodies\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 130kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using LRPPRC antibody at 1:1000 dilution.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\n Detection: ECL Basic Kit.\u003cbr\u003e\n Exposure time: 5s., Immunohistochemistry of paraffin-embedded human breast cancer using LRPPRC Rabbit pAb at dilution of 1:250. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded human colon carcinoma using LRPPRC Rabbit pAb at dilution of 1:250. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Confocal immunofluorescence analysis of Hela cells using LRPPRC Polyclonal Antibody at dilution of 1:400. Blue: DAPI for nuclear staining.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: LRPPRC; CLONE-23970; GP130; LRP130; LSFC\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human LRPPRC(NP_573566.2).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43052839141555,"sku":"BS70542-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43052839174323,"sku":"BS70542-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_07cba11d-93a0-4800-9087-657f35bb25b8.jpg?v=1708000089","url":"https:\/\/afsbio.com\/products\/lrpprc-polyclonal-antibody-bs70542","provider":"AFSBio Inc.","version":"1.0","type":"link"}