{"product_id":"m-rip-polyclonal-antibody-cas7516","title":"M-RIP Polyclonal Antibody-CAS7516","description":"\u003cp\u003eM-RIP Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: CAS7516-50, CAS7516-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q6WCQ1\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP,  IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:5000~1:10000\u003cbr\u003e\nIP:1:2000~1:8000\u003cbr\u003e\nIHC:1:50~1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: Myosin phosphatase-rho interacting protein (M-RIP), also known as p116RIP, RIP3, and MPRIP, localizes to actin-myosin filaments regulating cytoskeletal dynamics. M-RIP contains amino-terminal pleckstrin homology domains, carboxyl-terminal coiled-coil domains, and was originally identified to associate with the myosin phosphatase complex. M-RIP binds to MBS\/MYRT, the myosin binding subunit of myosin phosphatase, as well as RhoA. Phosphorylation of MYRT by Rho-associated kinase (ROCK) inhibits myosin phosphatase activity, resulting in increased levels of phosphorylation on myosin light chain, and enhanced contractility. M-RIP may function as a scaffolding protein for the complex between the myosin phosphatase complex, Rho\/ROCK, and actin. Silencing of M-RIP results in disassembly of the complex, increased phosphorylation of myosin light chain, and changes to cytoskeletal dynamics.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: M-RIP polyclonal antibody detects endogenous levels of M-RIP protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 130-160kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of M-RIP polyclonal antibody at 1:5000 dilution\u003cbr\u003e\n Lane 1: PC12 whole cell lysate (40ug)\u003cbr\u003e\n Lane 2: CT-26 whole cell lysate (40ug)\u003cbr\u003e\n Lane 3: H1792 whole cell lysate (40ug)\u003cbr\u003e\n Lane 4: Myla2059 whole cell lysate (40ug)\u003cbr\u003e\n Lane 5: PC3 whole cell lysate (40ug)\u003cbr\u003e\n Lane 6: SGC7901 whole cell lysate (40ug), Immunoprecipitation - M-RIP Polyclonal Antibody, Immunohistochemistry of paraffin-embedded human colon carcinoma using M-RIP antibody at dilution of 1:50., Immunohistochemistry of paraffin-embedded human colon carcinoma using M-RIP antibody at dilution of 1:50.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Myosin phosphatase Rho-interacting protein; \u003cbr\u003e\nM-RIP; Rho-interacting protein 3; RIP3; p116Rip; MPRIP\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant protein of human M-RIP.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43058776998067,"sku":"CAS7516-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43058777030835,"sku":"CAS7516-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_9f7afdd4-8b87-44b5-9b93-f1ccc1ad2a2a.jpg?v=1708172180","url":"https:\/\/afsbio.com\/products\/m-rip-polyclonal-antibody-cas7516","provider":"AFSBio Inc.","version":"1.0","type":"link"}