{"product_id":"map2-monoclonal-antibody-mb66861","title":"MAP2 Monoclonal Antibody-MB66861","description":"\u003cp\u003eMAP2 Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB66861-50, MB66861-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Mouse IgG1 kappa. Supplied in crude ascites with 0.01% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P11137\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB (1\/500 - 1\/1000), IHC (1\/50 - 1\/200), IF\/ICC (1\/10 - 1\/50)\u003c\/p\u003e\n\n\u003cp\u003eBackground: Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics, and organelle trafficking in axons and dendrites . Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B , and low molecular weight MAP2C and MAP2D . Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. GSK-3 and p44\/42 MAP kinase phosphorylate MAP2 at Ser136, Thr1620, and Thr1623 . Phosphorylation at Thr1620\/1623 by GSK-3 inhibits MAP2 association with microtubules and microtubule stability .\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of MAP2 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 59 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of MAP2 expression in MCF7 (A) whole cell lysates., Immunohistochemical analysis of MAP2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunofluorescent analysis of MAP2 staining in brain tissue cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Microtubule-associated protein 2; MAP-2\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human MAP2. The exact sequence is proprietary.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42989582844083,"sku":"MB66861-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42989582876851,"sku":"MB66861-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_c767fb88-e3b7-4437-8c30-6a05f2e3335c.jpg?v=1706095735","url":"https:\/\/afsbio.com\/products\/map2-monoclonal-antibody-mb66861","provider":"AFSBio Inc.","version":"1.0","type":"link"}