{"product_id":"mapk1-mapk3-polyclonal-antibody-bs80050","title":"MAPK1\/MAPK3 Polyclonal Antibody-BS80050","description":"\u003cp\u003eMAPK1\/MAPK3 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS80050-50, BS80050-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P28482\/P27361\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:1000 - 1:2000   |   IF\/ICC,1:100 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Unmodification\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: 41kDa\/43kDa\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic peptide of human MAPK1\/MAPK3(NP_620407.1\/NP_002737.2).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using MAPK1\/MAPK3 antibody at 1:1000 dilution.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% nonfat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Basic Kit.\u003cbr\u003e\nExposure time: 10s., Immunofluorescence analysis of NIH-3T3 cells using MAPK1\/MAPK3 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Confocal immunofluorescence analysis of Hela cells using MAPK1\/MAPK3 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42892340166835,"sku":"BS80050-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42892340199603,"sku":"BS80050-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_2568d7a7-28df-46a1-9af9-5c20668c5f3d.jpg?v=1702483310","url":"https:\/\/afsbio.com\/products\/mapk1-mapk3-polyclonal-antibody-bs80050","provider":"AFSBio Inc.","version":"1.0","type":"link"}