{"product_id":"mcl-1-rabbit-monoclonal-antibody-bs9891m","title":"Mcl-1 Rabbit Monoclonal Antibody-BS9891M","description":"\u003cp\u003eMcl-1 Rabbit Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS9891M-50, BS9891M-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q07820\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\/IF, IHC, FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:1000 \u003cbr\u003e\nICC\/IF: 1:50-1:200\u003cbr\u003e\nIHC: 1:50-1:200 \u003cbr\u003e\nFC: 1:50-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: B cell CLL\/lymphoma-2 (Bcl-2) blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Myeloid cell leukemia-1 (Mcl-1) shares sequence homology with Bcl-2 and further resembles Bcl-2 in that its expression promotes cell viability. p53 and Mcl-1 demonstrate opposing effects on mitochondrial apoptosis by mediating Bcl-2 antagonist killer (Bak) activity. Mcl-1 is an important and specific regulator that is necessary for the homeostasis of early hematopoietic progenitors. Glycogen synthase kinase-3 (GSK-3) controls Mcl-1 stability, which has an effect on the regulation of apoptosis by growth factors PI 3-kinase and Akt. Mice with a deficiency of the Mcl-1 protein show a significant reduction in B and T lymphocytes similar to the effects observed in IL-7- or IL-7R-deficient mice.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Protein A affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects endogenous levels of MCL1 and does not cross-react with related proteins.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 29, 37 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of MCL1 Rabbit mAb at 1:1000 dilution\u003cbr\u003e\n Lane 1: Raji whole cell lysate\u003cbr\u003e\n Lane 2: The heart tissue lysate of Mouse, Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MCL1 antibody.Counter stained with hematoxylin., ICC staining MCL1 in Hela cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Induced myeloid leukemia cell differentiation protein Mcl-1; Bcl-2-like protein 3; Bcl2-L-3; Bcl-2-related protein EAT\/mcl1; mcl1\/EAT; MCL1; BCL2L3\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant antibody.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43004765831347,"sku":"BS9891M-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43004765864115,"sku":"BS9891M-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_182eb408-cd1e-4e8d-9ac2-c1631dc36347.jpg?v=1706446025","url":"https:\/\/afsbio.com\/products\/mcl-1-rabbit-monoclonal-antibody-bs9891m","provider":"AFSBio Inc.","version":"1.0","type":"link"}