MCM3 Rabbit mAb
Sizes: 20µl, 100µl
Catalogue Numbers: A11475-20, A11475-100
Citations, Manuals and SDS Available upon request.
Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Several transcript variants encoding different isoforms have been found for this gene.
Synonyms: HCC5; P1.h; RLFB; P1-MCM3; MCM3
GeneID: 4172
Swiss Prot: P25205
Tested Applications: ELISA, WB, IHC-P, IF/ICC, IP
Reactivity: Human, Mouse, Rat
Calculated Molecular Weight: 91kDa
Observed Molecular Weight: 100kDa
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MCM3 (P25205).
Immunogen Species: Human
Host: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles.|Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Recommended Dilutions: WB: 1:500 - 1:1000 | IHC-P: 1:50 - 1:200 | IF/ICC: 1:50 - 1:200 | IP: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Research Areas: Epigenetics Nuclear Signaling, Cell Biology Developmental Biology, Cell Cycle, Centrosome
Modifications: Unmodified
Conjugate: Unconjugated
Positive Samples: HeLa, K-562, C6, Mouse spleen
Cellular Location: Nucleus
Keywords: MCM3, HCC5, P1-MCM3, P1.h, RLFB, minichromosome maintenance complex component 3, DNA replication licensing factor MCM3|DNA polymerase alpha holoenzyme-associated protein P1|DNA replication factor MCM3|MCM3 minichromosome maintenance deficient 3|RLF subunit beta|cervical cancer proto-oncogene 5|hRlf beta subunit|minichromosome maintenance deficient 3|p102|replication licensing factor, beta subunit, P25205
Symbol: MCM3
Category: Monoclonal Antibodies
Research Use Only