{"product_id":"mcu-polyclonal-antibody-bs90834","title":"MCU Polyclonal Antibody-BS90834","description":"\u003cp\u003eMCU Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90834-50, BS90834-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q8NE86(Human) Q3UMR5(Mouse)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC IHC FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500-1:1,000\u003cbr\u003e\nICC:1:50-1:200\u003cbr\u003e\nIHC:1:50-1:200\u003cbr\u003e\nFC:1:50-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Constitutes the pore-forming and calcium-conducting subunit of the uniporter complex. Activity is regulated by MICU1 and MICU2. At low Ca2+ levels MCU activity is down-regulated by MICU1 and MICU2; at higher Ca2+ levels MICU1 increases MCU activity. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes. While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Protein affinity purified.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: MCU polyclonal antibody detects endogenous levels of MCU protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: Predicted band size 40 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Fig1:Western blot analysis of MCU on different lysates using anti-MCU antibody at 1\/500 dilution.\u003cbr\u003e\n Positive control:\u003cbr\u003e\n Lane 1: A549 \u003cbr\u003e\n Lane 3: mouse spleen tissue\u003cbr\u003e\n Lane 2: HL-60 \u003cbr\u003e\n Lane 4: mouse brain tissue, ICC staining MCU in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: HsMCU, MCU, C10orf42, CCDC109A, C109A, C10orf42, Calcium uniporter protein mitochondrial, Ccdc109a, Coiled-coil domain-containing protein 109A, HsMCU, Mitochondrial Calcium Uniporter,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: Recombinant protein within human MCU aa 50-250.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42984591327411,"sku":"BS90834-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42984591360179,"sku":"BS90834-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_646b95eb-9ca6-4a51-97b1-e01ce73a6ac6.jpg?v=1705918906","url":"https:\/\/afsbio.com\/products\/mcu-polyclonal-antibody-bs90834","provider":"AFSBio Inc.","version":"1.0","type":"link"}