{"product_id":"med18-v102-polyclonal-antibody-bs3141","title":"MED18 (V102) Polyclonal Antibody-BS3141","description":"\u003cp\u003eMED18 (V102) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS3141-50, BS3141-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Mediator of RNA polymerase II transcription subunit 18; Mediator complex subunit 18; p28b; MED18\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9BUE0\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003c\/p\u003e\n\n\u003cp\u003eBackground: In mammalian cells, transcription is regulated in part by high molecular weight co-activating complexes that mediate signals between transcriptional activators and RNA polymerase II (Pol II). The mediator complex is one such multi-protein structure that functions as a bridge between regulatory proteins and Pol II, thereby regulating Pol II-dependent transcription. Med18 (mediator complex subunit 18), also known asp28b, is a 208 amino acid protein that localizes to nucleus and exists as a component of the mediator complex. Working in tandem with several other proteins, including Med8 and Med25, Med18 serves as a scaffold for the assembly of a functional preinitiation complex with Pol II and general transcription factors, thereby activating the transcription of Pol II-dependent genes.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: MED18 (V102) polyclonal antibody detects endogenous levels of MED18 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 24 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of MED18 (V102) pAb at 1:1000 dilution\u003cbr\u003e\nLane 1: HepG2 whole cell lysate(20ug)\u003cbr\u003e\nLane 2: SGC7901 whole cell lysate(20ug)\u003cbr\u003e\nLane 3: The Brain tissue lysate of Rat(40ug)\u003cbr\u003e\nLane 4: The Brain tissue lysate of Mouse(40ug)\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 72-120 of Human MED18.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42911802130611,"sku":"BS3141-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42911802163379,"sku":"BS3141-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_61654c95-c354-45de-844b-f3f03b9e5565.jpg?v=1703179500","url":"https:\/\/afsbio.com\/products\/med18-v102-polyclonal-antibody-bs3141","provider":"AFSBio Inc.","version":"1.0","type":"link"}