{"product_id":"mekk3-polyclonal-antibody-bs90845","title":"MEKK3 Polyclonal Antibody-BS90845","description":"\u003cp\u003eMEKK3 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90845-50, BS90845-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q99759(Human) Q61084(Mouse) Unigene:72680(Rat)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\/IF, IHC, IP, , FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:1,000-1:2,000\u003cbr\u003e\nICC:1:100-1:500\u003cbr\u003e\nIHC:1:50-1:200\u003cbr\u003e\nFC:1:50-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: MAP kinase kinase kinase (MEKK3 or MAP3K3) is a serine\/threonine protein kinase that activates SAPK and ERK via phosphorylation and activation of their respective MAP kinase kinases, SEK and MEK1\/2. MEKK3 also stimulates MEK5 via activation of ERK5\/BMK1, which is at least partly regulated by a direct interaction between MEK5 and MEKK3 via p67phox-Bem1p (PB1) protein-protein interaction domains found in both proteins. MEKK3 modulates NF-κB activation in response to a variety of agonists including TNFα, LPS, IL-1 and LPA. Despite reports showing that phosphorylation of MEKK3 at Ser526 within the activation loop is necessary for kinase activation, at least one study suggests that dual phosphorylation at Thr516 and Ser520 is required for LPA-stimulated IKKβ\/NF-κB activation. Phosphorylation at Thr294 appears to negatively regulate MEKK3 by promoting 14-3-3β binding and inhibition of the kinase activity.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProA affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: MEKK3 polyclonal antibody detects endogenous levels of MEKK3 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 71 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of MEKK3 on different lysates using anti-MEKK3 antibody at 1\/1,000 dilution. \u003cbr\u003e\n Positive control: \u003cbr\u003e\n Lane 1: Hela \u003cbr\u003e\n Lane 2: 293T \u003cbr\u003e\n Lane 3: A431, ICC staining MEKK3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: MEK kinase 3, MEKK 3, MAP3K3, M3K3, MAP\/ERK kinase kinase 3, MAP3K3, MAPK\/ERK kinase kinase 3, MAPKKK3, MEK kinase 3, MEKK 3, MEKK3, Mitogen activated protein kinase kinase kinase 3, Mitogen-activated protein kinase kinase kinase 3,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: recombinant protein\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42984592375987,"sku":"BS90845-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42984592408755,"sku":"BS90845-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_2018ba50-799a-4aac-991e-a981ceb07359.jpg?v=1705918935","url":"https:\/\/afsbio.com\/products\/mekk3-polyclonal-antibody-bs90845","provider":"AFSBio Inc.","version":"1.0","type":"link"}