{"product_id":"mettl13-polyclonal-antibody-bs74918","title":"METTL13 Polyclonal Antibody-BS74918","description":"\u003cp\u003eMETTL13 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS74918-50, BS74918-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q8N6R0\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eMETTL13 Polyclonal Antibody-BS74918\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000  |  IF\/ICC,1:50 - 1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: Dual methyltransferase that catalyzes methylation of elongation factor 1-alpha (EEF1A1 and EEF1A2 at two different positions, and is therefore involved in the regulation of mRNA translation. Via its C-terminus, methylates EEF1A1 and EEF1A2 at the N-terminal residue 'Gly-2'. Via its N-terminus dimethylates EEF1A1 and EEF1A2 at residue 'Lys-55'. Has no activity towards core histones H2A, H2B, H3 and H4. Negatively regulates cell proliferation at G1\/S transition via transcriptional suppression of cell cycle regulatory genes such as CDK4 and CDK6.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Unmodification\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 85kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of MCF7 cells, using METTL13 antibody at 1:1000 dilution.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Enhanced Kit.\u003cbr\u003e\nExposure time: 30s., Immunofluorescence analysis of A549 cells using METTL13 antibody. Blue: DAPI for nuclear staining.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: METTL13; 5630401D24Rik; CGI-01; KIAA0859; feat\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human METTL13(NP_057019.3).\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42957347848371,"sku":"BS74918-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42957347881139,"sku":"BS74918-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_5e9a2290-0a80-4b14-936d-b15a39c770c4.jpg?v=1705145150","url":"https:\/\/afsbio.com\/products\/mettl13-polyclonal-antibody-bs74918","provider":"AFSBio Inc.","version":"1.0","type":"link"}