{"product_id":"mglur-5-k56-polyclonal-antibody-bs2732","title":"mGluR-5 (K56) Polyclonal Antibody-BS2732","description":"\u003cp\u003emGluR-5 (K56) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS2732-50, BS2732-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P41594\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\u003c\/p\u003e \n\n\u003cp\u003eBackground: The mGluR proteins (metabotropic glutamate receptors) are members of the G protein-coupled receptor family and are functionally and pharmacologically distinct from the GluR proteins (ionotropic glutamate receptors). The eight currently known mGluR proteins are mediated by two G proteins with opposing regulation of adenylate cyclase pathways. The activities of mGluR-1 and mGluR-5 are mediated by a G protein that activates a phosphatidylinositolcalcium second messenger system and generates a calcium-activated chloride current. The remainder of the eight subtypes of mGluR have an activity mediated by a G protein that inhibits adenylate cyclase activity. mGluR-3, which may interact with GRASP, acts as a receptor for glutamate.\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: mGluR5 (K56) polyclonal antibody detects endogenous levels of mGluR5 protein.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of mGluR-5 (K56) pAb at 1:500 dilution\u003cbr\u003e\nLane 1: C6 whole cell lysate (40ug)\u003cbr\u003e\nLane 2: BV2 whole cell lysate (40ug)\u003cbr\u003e\nLane 3: HEK293T whole cell lysate (10ug)\u003cbr\u003e\nLane 4: SK-OVCAR3 whole cell lysate (40ug)\u003cbr\u003e\nLane 5: L02 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of mGluR-5 (K56) pAb in paraffin-embedded human esophageal carcinoma tissue at 1:50.showing cell membrane staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: ~132 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Metabotropic glutamate receptor 5; mGluR5; GRM5; GPRC1E; MGLUR5\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 30-80 of Human mGluR-5.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42953229074611,"sku":"BS2732-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42953229107379,"sku":"BS2732-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_cd65de48-f3e5-412e-ad91-658bd552133a.jpg?v=1705057238","url":"https:\/\/afsbio.com\/products\/mglur-5-k56-polyclonal-antibody-bs2732","provider":"AFSBio Inc.","version":"1.0","type":"link"}