{"product_id":"mindin-w104-polyclonal-antibody-bs9256","title":"Mindin (W104) Polyclonal Antibody-BS9256","description":"\u003cp\u003eMindin (W104) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS9256-50, BS9256-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9BUD6\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003c\/p\u003e\n\n\u003cp\u003eBackground: The Thrombospondin proteins, Thrombospondins 1-4 and Thrombospondin 5 (also designated COMP), compose a family of glycoproteins that are involved in cell-to-cell and cell-to-matrix signaling. These extracellular, cell-surface proteins form complexes of both homo- and heteromultimers. Spondin-2, or Mindin, is also designated DIL-1 for its differential expression in cancerous and non-cancerous lung cells. Full-length SPON2 cDNA encodes a 331 amino acid protein with a domain arrangement similar to zebrafish F-Spondin and Mindin-1\/Mindin-2: an FS1 domain, an FS2 domain, a hydrophobic signal sequence in the N-terminus and a Thrombospondin type I repeat. Immunoblot analysis demonstrates expression of a 42 kDa protein under reducing conditions and expression of dimers and oligomers in a concentration-dependent manner under nonreducing conditions.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Mindin (W104) polyclonal antibody detects endogenous levels of Mindin protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of SPON2 pAb at 1:2000 dilution\u003cbr\u003e\nLane1:PC12 whole cell lysate(40ug)\u003cbr\u003e\nLane2:LOVO whole cell lysate(40ug)\u003cbr\u003e\nLane3:SGC7901 whole cell lysate(40ug)\u003cbr\u003e\nLane4:HEK293T whole cell lysate(30ug)\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 35 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: DIL 1; DIL-1; DIL1; M spondin; Mindin; SPON 2; Spon2; Spondin 2; Spondin-2;\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 70-118 of Human Mindin.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42910468931763,"sku":"BS9256-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42910468964531,"sku":"BS9256-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_c0d65ce7-74db-49ec-ab4c-ba298f4eebf4.jpg?v=1703093001","url":"https:\/\/afsbio.com\/products\/mindin-w104-polyclonal-antibody-bs9256","provider":"AFSBio Inc.","version":"1.0","type":"link"}