{"product_id":"mini-samples-elisa-kit-for-amylin-mea812ra","title":"Mini Samples ELISA Kit for Amylin - MEA812Ra","description":"\u003cp\u003eMini Samples ELISA Kit for Amylin\u003c\/p\u003e\n\n\u003cp\u003eSizes: 24T, 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MEA812Ra-24, MEA812Ra-48, MEA812Ra-96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eFeatured Series: Mini samples kit\u003c\/p\u003e\n\n\u003cp\u003eTarget: Amylin\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: IAPP; IAP; DAP; Islet Amyloid Polypeptide; Insulinoma Amyloid Polypeptide; Diabetes-associated peptide\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Rat\u003c\/p\u003e\n\n\u003cp\u003eUniprot: P12969\u003c\/p\u003e\n\n\u003cp\u003eGene ID: 24476\u003c\/p\u003e\n\n\u003cp\u003eFeatured Series Function: Detects protein (micro-volume samples 15-25 μl)\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Reactive with Rat Amylin \/ Amylin\u003c\/p\u003e\n\n\u003cp\u003eMethod: Colormetric\u003c\/p\u003e\n\n\u003cp\u003eDetection principle: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eDetection\u003cbr\u003e\nrange: 12.35-1,000pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 4.37pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eAssay Time: 3h\u003c\/p\u003e\n\n\u003cp\u003eSample Size: 15-25uL\u003c\/p\u003e\n\n\u003cp\u003eRecommended\/Predicted\u003cbr\u003e\nSample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Precision: Intra-Assay: CV\u0026lt;10%, Inter-Assay: CV\u0026lt;12%\u003c\/p\u003e\n\n\u003cp\u003eStorage: 4°C for 1 month\/ -20°C for long-term(One year within shelf life)\u003c\/p\u003e\n\n\u003cp\u003eShelf-life: 12 months\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Amylin.\u003cbr\u003e\nNo significant cross-reactivity or interference between Mini Samples Amylin and analogues was observed.\u003c\/p\u003e\n\n\u003cp\u003ePrecision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Amylin were tested 20 times on one plate, respectively.\u003cbr\u003e\nInter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Amylin were tested on 3 different plates, 8 replicates in each plate.\u003cbr\u003e\nCV(%) = SD\/meanX100\u003c\/p\u003e\n\n\u003cp\u003eStability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.\u003cbr\u003e\nTo minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\u003c\/p\u003e\n\n\u003cp\u003eTarget: 1. Prepare all reagents, samples and standards;\u003cbr\u003e\n2. Add 25µL standard or sample to each well.\u003cbr\u003e\n And then add 25μL prepared Detection Reagent A immediately.\u003cbr\u003e\n Shake and mix. Incubate 1 hour at 37°C;\u003cbr\u003e\n3. Aspirate and wash 3 times;\u003cbr\u003e\n4. Add 50µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;\u003cbr\u003e\n5. Aspirate and wash 5 times;\u003cbr\u003e\n6. Add 50µL Substrate Solution. Incubate 10-20 minutes at 37°C;\u003cbr\u003e\n7. Add 25µL Stop Solution. Read at 450 nm immediately.\u003c\/p\u003e\n\n\u003cp\u003eTest principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Amylin has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Amylin and unlabeled Mini Samples Amylin (Standards or samples) with the pre-coated antibody specific to Mini Samples Amylin. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Amylin in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Amylin in the sample.\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Metabolic pathway; Endocrinology; Hepatology;\u003c\/p\u003e \n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Cloud-Clone","offers":[{"title":"24T","offer_id":45379565912243,"sku":"MEA812Ra-24","price":459.2,"currency_code":"CAD","in_stock":true},{"title":"48T","offer_id":45379565945011,"sku":"MEA812Ra-48","price":805.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45379565977779,"sku":"MEA812Ra-96","price":1149.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Cloud-Clone.jpg?v=1774117629","url":"https:\/\/afsbio.com\/products\/mini-samples-elisa-kit-for-amylin-mea812ra","provider":"AFSBio Inc.","version":"1.0","type":"link"}