{"product_id":"mini-samples-elisa-kit-for-nuclear-factor-kappa-b-nfkb-meb824mu","title":"Mini Samples ELISA Kit for Nuclear Factor Kappa B (NFkB) - MEB824Mu","description":"Mini Samples ELISA Kit for Nuclear Factor Kappa B (NFkB)\n\nSizes: 24T, 48T, 96T\n\nCatalogue Numbers: MEB824Mu-24, MEB824Mu-48, MEB824Mu-96\n\nCitations, Manuals and MSDS Available upon request.\n\nFeatured Series: Mini samples kit\n\nTarget: Nuclear Factor Kappa B\n\nAlternative Names: NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit\n\nTarget Species: Mouse\n\nUniprot: P25799\n\nGene ID: 18033\n\nFeatured Series Function: Detects protein (micro-volume samples 15-25 μl)\n\nSpecificity: Reactive with Mouse NFkB \/ Nuclear Factor Kappa B\n\nMethod: Colormetric\n\nDetection principle: Double-antibody Sandwich\n\nDetection\nrange: 0.156-10ng\/mL\n\nSensitivity: 0.059ng\/mL\n\nAssay Time: 3h\n\nSample Size: 15-25uL\n\nRecommended\/Predicted\nSample Types: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids\n\nAssay Precision: Intra-Assay: CV\u0026lt;10%, Inter-Assay: CV\u0026lt;12%\n\nStorage: 4°C for 1 month\/ -20°C for long-term(One year within shelf life)\n\nShelf-life: 12 months\n\nSpecificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Nuclear Factor Kappa B (NFkB).\nNo significant cross-reactivity or interference between Mini Samples Nuclear Factor Kappa B (NFkB) and analogues was observed.\n\nPrecision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Nuclear Factor Kappa B (NFkB) were tested 20 times on one plate, respectively.\nInter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Nuclear Factor Kappa B (NFkB) were tested on 3 different plates, 8 replicates in each plate.\nCV(%) = SD\/meanX100\n\nStability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.\nTo minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\n\nTarget: 1. Prepare all reagents, samples and standards;\n2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;\n3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;\n4. Aspirate and wash 3 times;\n5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;\n6. Aspirate and wash 5 times;\n7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;\n8. Add 20µL Stop Solution. Read at 450nm immediately.\n\nTest principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Nuclear Factor Kappa B (NFkB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Nuclear Factor Kappa B (NFkB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Nuclear Factor Kappa B (NFkB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Nuclear Factor Kappa B (NFkB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.\n\nResearch Area: Signal transduction; \n\nResearch Use Only","brand":"Cloud-Clone","offers":[{"title":"24T","offer_id":45379846471859,"sku":"MEB824Mu-24","price":386.4,"currency_code":"CAD","in_stock":true},{"title":"48T","offer_id":45379846504627,"sku":"MEB824Mu-48","price":677.6,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45379846537395,"sku":"MEB824Mu-96","price":967.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Cloud-Clone.jpg?v=1774117629","url":"https:\/\/afsbio.com\/products\/mini-samples-elisa-kit-for-nuclear-factor-kappa-b-nfkb-meb824mu","provider":"AFSBio Inc.","version":"1.0","type":"link"}