{"product_id":"mklp1-rabbit-monoclonal-antibody-bs9910m","title":"MKLP1 Rabbit Monoclonal Antibody - BS9910M","description":"\u003cp\u003eMKLP1 Rabbit Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS9910M-50, BS9910M-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q02241\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\/IF\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:1000-1:5000 \u003cbr\u003e\nICC\/IF: 1:50-1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: MKLP-1 (mitotic kinesin-like protein-1), represents a novel kinesin. Sequence analysis has also been shown to predict that MKLP-1 possesses features typical of nuclear proteins. Immunocytological studies have demonstrated that MKLP-1 moves from the nucleus early in mitosis and then to the midbody after cytokinesis. MKLP-1 has been shown to bundle antiparallel microtubules in vitro and to move them at rates comparable to spindle elongation in vivo. A hamster homolog of MKLP-1, designated CHO1 antigen, has also been isolated. Although apparently functionally equivalent with respect to microtubule bundling activity, there are significant differences between the human and hamster proteins at their C-termini, possibly due to alternative splicing or the presence of more than one MKLP-1 locus.\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Protein A affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects endogenous levels of MKLP1 and does not cross-react with related proteins.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of MKLP1 Rabbit mAb at 1:1000 dilution\u003cbr\u003e\nLane1:A549 whole cell lysate\u003cbr\u003e\nLane2:Hela whole cell lysate, ICC staining MKLP1 in Hela cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant antibody.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42892014583987,"sku":"BS9910M-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42892014616755,"sku":"BS9910M-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_3c46f61f-18a9-4cb3-8403-5090a5571635.jpg?v=1702469205","url":"https:\/\/afsbio.com\/products\/mklp1-rabbit-monoclonal-antibody-bs9910m","provider":"AFSBio Inc.","version":"1.0","type":"link"}