{"product_id":"mlh1-monoclonal-antibody-mb0151","title":"MLH1 Monoclonal Antibody-MB0151","description":"\u003cp\u003eMLH1 Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB0151-50, MB0151-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P40692\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:500~1000\u003cbr\u003e\nICC: 1:50~200\u003c\/p\u003e\n\n\u003cp\u003eBackground: MLH1 is an integral part of the protein complex responsible for mismatch repair that is expressed in lymphocytes, heart, colon, breast, lung, spleen, testis, prostate, thyroid and gall bladder and is methylated in several ovarian tumors. Loss of MLH1 protein expression is associated with a mutated phenotype, microsatellite instability and a predisposition to cancer. In hereditary nonpolyposis colorectal cancer (HNPCC), an autosomal dominant inherited cancer syndrome that signifies a high risk of colorectal and various other types of cancer, the MLH1 gene exhibits a pathogenic mutation. Certain cancer cell lines, including leukemia CCRF-CEM, colon HCT 116 and KM12, and ovarian cancers SK-OV-3 and IGROV-1, show complete deficiency of MLH1, while MLH1 is expressed in 60% of melanomas, 70% of noninvasive squamous cell carcinomas and 30% of invasive squamous cell carcinomas.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects endogenous levels of MLH1 and does not cross-react with related proteins\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: Predicted band size: 85KDa\u003cbr\u003e\nObserved band size: 85KDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot detection of MLH1 antibody in SW480, 293T, Jurkat and Hela cell lysates using MLH1 antibody (1:500 diluted)., Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and using anti-MLH1 antibody (dilution 1:100).\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: DNA mismatch repair protein Mlh1; MutL protein homolog 1; COCA2\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant full length Human MLH1.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42989566918835,"sku":"MB0151-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42989566951603,"sku":"MB0151-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_34f836a5-513b-4d12-9d6b-cbc1e94de5cd.jpg?v=1706095484","url":"https:\/\/afsbio.com\/products\/mlh1-monoclonal-antibody-mb0151","provider":"AFSBio Inc.","version":"1.0","type":"link"}