{"product_id":"mlk3-phospho-t277-s281-polyclonal-antibody-bs64014","title":"MLK3 (phospho-T277\/S281) Polyclonal Antibody - BS64014","description":"\u003cp\u003eMLK3 (phospho-T277\/S281) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μl, 100μl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS64014-50, BS64014-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: Mixed lineage kinase 3 (MLK3) is a serine\/threonine kinase that has an amino-terminal SH3 domain followed by the kinase domain and two leucine zippers, a cdc42\/Rac1 binding (CRIB) domain and several other domains\/motifs at the carboxy-terminal region. CRIB triggers the dimerization of MLK3 via its tandem leucine zippers, followed by the intramolecμlar phosphorylation and subsequent activation of MLK3. Autophosphorylation of Thr277 and Ser281 is essential for MLK3 kinase activity. Ser281 is also phosphorylated by HPK in an in vitro kinase assay. MLK3 functions as a MAPKKK of the SAPK\/JNK stress pathway by directly phosphorylating SEK1\/MKK4 and MKK7, although it is controversial whether MLK3 is involved in p38 stress pathway activation. MLK3 also functions as an IκB kinase and mediates the activation of the transcriptional factor NF-κB stimμlated by CD3\/CD28, suggesting a role for MLK3 in immune and inflammatory responses.\u003c\/p\u003e\n\n\u003cp\u003eCategory: Primary Antibody\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: Mitogen-activated protein kinase kinase kinase 11, Mixed lineage kinase 3, Src-homology 3 domain-containing proline-rich kinase, MAP3K11, MLK3, PTK1, SPRK\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic phosphopeptide derived from human MLK3 around the phosphorylation site of Threonine 277 \/ Serine 281.\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB: 1:1000~1:2000\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: MLK3(Phospho-T277\/S281) polyclonal antibody detects endogenous levels of MLK3 protein only when phosphorylated at Thr277\/Ser281.\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: ~ 92 kDa\u003c\/p\u003e\n\n\u003cp\u003ePathway: Signaling Pathways Activating p38 MAP Kinase,\u003c\/p\u003e \n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSwissProt: Q16584\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Bioworld","offers":[{"title":"50μl","offer_id":45946342375603,"sku":"BS64014-50","price":289.5,"currency_code":"CAD","in_stock":true},{"title":"100μl","offer_id":45946342408371,"sku":"BS64014-100","price":492.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Bioworld_Antibody_Image_6ca33534-2486-4e91-ac84-9e0d75778258.jpg?v=1788363539","url":"https:\/\/afsbio.com\/products\/mlk3-phospho-t277-s281-polyclonal-antibody-bs64014","provider":"AFSBio Inc.","version":"1.0","type":"link"}