{"product_id":"mmp9-cleaved-phe107-rabbit-polyclonal-antibody-ra27309","title":"MMP9 (Cleaved-Phe107) Rabbit Polyclonal Antibody - RA27309","description":"\u003cp\u003eMMP9 (Cleaved-Phe107) Rabbit Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RA27309-50, RA27309-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: catalytic activity:Cleavage of gelatin types I and V and collagen types IV and V.,cofactor:Binds 2 zinc ions per subunit.,cofactor:Binds 3 calcium ions per subunit.,disease:Defects in MMP9 may be a cause of susceptibility to lumbar disk herniation (LDH) [MIM:603932]. LDH is the predominant cause of low-back pain and unilateral leg pain.,domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.,enzyme regulation:Inhibited by histatin-3 1\/24 (histatin-5) .,function:May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.,induction:Activated by 4-aminophenylmercuric acetate and phorbol ester.,miscellaneous:In the arthritis patient this enzyme might contribute to the pathogenesis of joint destruction and might constitute a useful marker of disease status.,PTM:N- and O-glycosylated.,PTM:Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.,similarity:Belongs to the peptidase M10A family.,similarity:Contains 3 fibronectin type-II domains.,similarity:Contains 4 hemopexin-like domains.,subunit:Exists as monomer, disulfide-linked homodimer, and as a heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form.,tissue specificity:Produced by normal alveolar macrophages and granulocytes.,\u003c\/p\u003e\n\n\u003cp\u003eCondition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eStorage Instructions: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eRecommended dilutions: WB 1:1000-2000 ELISA 1:5000-20000\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: Matrix metalloproteinase-9 (MMP-9;EC 3.4.24.35;92 kDa gelatinase;92 kDa type IV collagenase;Gelatinase B;GELB) [Cleaved into: 67 kDa matrix metalloproteinase-9; 82 kDa matrix metalloproteinase-9]\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB; ELISA\u003c\/p\u003e\n\n\u003cp\u003eSpecies Cross-Reactivity: Human; Rat; Mouse\u003c\/p\u003e\n\n\u003cp\u003eGeneID (Human): 4318\u003c\/p\u003e\n\n\u003cp\u003eProtein MW (KDa): 66 78\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P14780\u003c\/p\u003e\n\n\u003cp\u003eSource: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Reed Bio","offers":[{"title":"50μL","offer_id":45121400176819,"sku":"RA27309-50","price":336.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":45121400209587,"sku":"RA27309-100","price":448.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/logo_1f0fc9e5-e71f-4486-a9d9-6aa56a7a7158.png?v=1773347244","url":"https:\/\/afsbio.com\/products\/mmp9-cleaved-phe107-rabbit-polyclonal-antibody-ra27309","provider":"AFSBio Inc.","version":"1.0","type":"link"}